Summary
Comparative studies on growth kinetics of cultivated human hair bulb papilla cells (PCs) and hair root sheath fibroblasts (RSFs) yielded evidence of some pecularities of PCs in both proliferative behavior and morphological growth pattern. As the dermal papilla, essentially supporting the nutrition of matrix epithelium, can be considered a target tissue for agents influencing maintenance of hair growth, we studied the effects of epidermal growth factor (EGF), fibroblasts growth factor (FGF), minoxidil (Mino), and hydrocortisone (HC) on the proliferation of PCs and RSFs, both gained from dissected hair follicles of scalp biopsy specimens of two male adults and separately cultured in vitro. EGF and FGF proved to increase proliferation of both PCs and RSFs most, yet at a different intensity for each cell group. HC slowed proliferation, and Mino failed to influence growth of PCs and RSFs.
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Arnold T, Deinlein E (1985) Prinzip der DNS-Cytometrie zur Bestimmung des nucleären DNS-Gehaltes von Zellkernsuspensionen. Anwendungsmöglichkeiten mittels Ethidiumbromid/Mithramycin-Fluorochromierung. Zentralbl Haut Geschlkrankh 150:727–741
Baden HP, Kubilus J (1983) Effect of minoxidil on cultured keratinocytes. J Invest Dermatol 81:558–560
Baisch H, Göhde W, Linden WA (1975) Analysis of PCP-data to determine the fraction of cells in the various phases of the cell cycle. Radiat Environ Biophys 12:31–39
Cohen J (1961) The transplantation of individual rat and guinea-pig whisker papillae. J Embryol Exp Morphol 9:117–127
Deinlein E, Schell H, Winter M, Hornstein OP, Katsuoka K (1985) DNA flow cytometry: a new technique for rapid analysis of cell-cycle kinetics in human anagen hairs. Arch Dermatol Res 277:337–339
Jahoda CAB, Oliver RF (1981) The growth of vibrissa dermal papilla cells in vitro. Br J Dermatol 105:623–627
Jahoda CAB, Oliver RF (1984) Vibrissa dermal papilla aggregative behaviour in vivo and in vitro. J Embryol Exp Morphol 79:211–224
Jones KL, Addison J (1976) Studies of human diploid fibroblast growth. I. Responses of normal and hypopituitary cells to fibroblast growth factor, insulin and serum. J Clin Endocrinol Metab 43:721–729
Kamely D, Rudland PS (1976) Induction of DNA synthesis and cell division in human diploid skin fibroblasts by fibroblast growth factor. Exp Cell Res 97:120–126
Katsuoka K, Schell H, Hornstein OP, Deinlein E, Wessel B (1986) Comparative morphological and growth kinetics studies on human hair bulb papilla cells and root sheath fibroblasts in vitro. Arch Dermatol Res 279:20–25
Kollar EJ (1970) The induction of hair follicles by embryogenic dermal papillae. J Invest Dermatol 55:374–378
Lembach KJ (1976) Induction of human fibroblast proliferation by epidermal growth factor (EGF): enhancement by an EGF-binding arginine esterase and by ascorbate. Proc Natl Acad Sci USA 73:183–187
Messenger AG (1984) The culture of dermal papilla cells from human hair follicles. Br J Dermatol 110:685–689
Messenger AG, Jennifer H sen, Blechen SS (1986) The in vitro properties of dermal papilla cell lines established from human hair follicles. Br J Dermatol 114:425–430
Moore GPM, Panaretto BA, Robertson D (1981) Effects of epidermal growth factor on hair growth in the mouse. J Endocrinol 88:293–299
Oliver RF (1966) Whisker growth after removal of the dermal papilla and lengths of the follicle in the hooded rat. J Embrol Exp Morphol 15:331–347
Oliver RF (1970) The induction of follicle formation in the adult hooded rat by vibrissa dermal papillae. J Embryol Exp Morphol 23:219–236
Oliver RF (1976) The experimental induction of whisker growth in the hooded rat by implantation of dermal papillae. J Embryol Exp Morphol 18:43–51
Ponec M, de Haas C, Bachra BN, Polano MK (1977) Effects of glucocorticosteroids on primary human fibroblasts. I. Inhibition of the proliferation of cultured primary human skin and mouse L 929 fibroblasts. Arch Dermatol Res 259:117–123
Weiss VC, West DP (1985) Topical minoxidil therapy and hair regrowth. Arch Dermatol 121:191–192
Zante J, Schumann J, Barlogie B, Göhde W, Büchner T (1976) New staining and preparation procedure for specific and rapid analysis of DNA distribution. In: Göhde W, Schumann J, Büchner R (eds) 2nd International symposium on pulse cytophotometry. European Press Medikon, Ghent, pp 97–106
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Katsuoka, K., Schell, H., Wessel, B. et al. Effects of epidermal growth factor, fibroblast growth factor, minoxidil and hydrocortisone on growth kinetics in human hair bulb papilla cells and root sheath fibroblasts cultured in vitro. Arch Dermatol Res 279, 247–250 (1987). https://doi.org/10.1007/BF00417323
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DOI: https://doi.org/10.1007/BF00417323