Summary
The identification of the entire structure of the Thormählen-positive compound B from melanotic urine is described. The compound was separated from other Thormählen-positive compounds using DEAE-cellulose column chromatography. On the basis of differential enzymic hydrolysis followed by gas chromatography-mass spectrometry analysis and comparison with synthetically prepared compounds it is pissible to conclude that the Thormählen-positive compound B is a mixture of O-sulphate 5-hydroxy-6-methoxyindole and 6-hydroxy-5-methoxyindole with predominance of the latter.
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Supported by grant No. 82-10 GUKC from Koningin Wilhelmina Fonds (Netherlands Cancer Foundation)
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Pavel, S., Boverhof, R. & van der Slik, W. Identification of Thormählen-positive compound “B” in urine of patients with malignant melanoma. Arch Dermatol Res 276, 156–159 (1984). https://doi.org/10.1007/BF00414012
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DOI: https://doi.org/10.1007/BF00414012