Skip to main content
Log in

The ultrastructure of vegetative cells and cysts of Azotobacter chroococcum

  • Published:
Archiv für Mikrobiologie Aims and scope Submit manuscript

Summary

The ultrastructure of vegetative cells and cysts of Azotobacter chroococcum shows that these two morphological forms are distinctly different. The cyst is surrounded by a multi-layered envelope consisting of the following structural elements: an irregularly outlined exocystorium and a cyst coat made up of an outer dense and an inner less dense layer. The cell inside the cyst coat shows essentially the same structural features as the normal vegetative cell.

The cyst thus bears no true resemblance to the endospore of the Grampositive bacillus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Birch-Andersen, A.: The use of epoxy resins as embedding media for electron microscopy. Proc. 4th. Int. Conf. Electron Microscopy Berlin Sept., 1958, II, pp. 44–52. Berlin, Göttingen, Heidelberg: Springer 1960.

    Google Scholar 

  • Bisset, K. A., and C. M. F. Hale: The cytology and life-cycle of Azotobacter chroococcum. J. gen. Microbiol. 8, 442–448 (1953).

    PubMed  Google Scholar 

  • Bisset, K. A.: Evidence from the cytology of Azotobacter chroococcum of a relationship with Rhizobium and the Bacillaceae. J. gen. Microbiol. 13, 442–445 (1955).

    PubMed  Google Scholar 

  • Bisset, K. A., A. C. Baird-Parker, C. M. F. Hale, M. H. Jeynes and J. C. Lawrence: The production of Gram-positive variants by Azotobacter chroococcum. J. gen. Microbiol. 16, 572–575 (1957).

    PubMed  Google Scholar 

  • Carr, L. B.: Electron microscopy of bacteria. Bact. Proc. 32 (1957).

  • Chapman, G. B., and K. A. Zworykin: Study of germinating Bacillus cereus spores employing television microscopy of living cells and electron microscopy of ultra-thin sections. J. Bact. 74, 126–132 (1957).

    PubMed  Google Scholar 

  • Davis, J. M.: Preparation and sectioning of tissues embedded in “Araldite” for electron microscope examination. Nature (Lond.) 183, 200–202 (1959).

    Google Scholar 

  • Fitz-James, P. C.: Participation of the cytoplasmic membrane in the growth and spore formation of bacilli. J. biophys. biochem. Cytol. 8, 507–528 (1960).

    Google Scholar 

  • Floethmann, E.: Cytologische Untersuchungen an Azotobacter chroococcum Beij. Arch. Mikrobiol. 20, 243 (1954).

    PubMed  Google Scholar 

  • Glauert, A. M., and R. H. Glauert: Araldite as an embedding medium for electron microscopy. J. biophys. biochem. Cytol. 4, 191–199 (1958).

    PubMed  Google Scholar 

  • Glauert, A. M., and D. A. Hopwood: A membranous component of the cytoplasm in Streptomyces coelicolor. J. biophys. biochem. Cytol. 6, 515 (1959).

    PubMed  Google Scholar 

  • Glauert, A. M., and D. A. Hopwood: The fine structure of Streptomyces coelicolor. I. The cytoplasmic membrane system. J. biophys. biochem. Cytol. 7, 479–488 (1960).

    PubMed  Google Scholar 

  • Hale, C. M. F., and K. A. Bisset: A comparison of the staining reactions of the cell walls of Azotobacter chroococcum and those of Gram-positive and Gramnegative bacteria. J. gen. Microbiol. 15, 423–427 (1956).

    PubMed  Google Scholar 

  • Iterson, W. van: Membranes, particular organelles and peripheral bodies in bacteria. Proc. Eur. Reg. Conf. on Electron Microscopy, Delft 1960, Vol. II, pp. 763–768.

  • Iterson, W. van: Some features of a remarkable organelle in Bacillus subtilis. J. biophys biochem. Cytol. 9 183–192 (1961).

    PubMed  Google Scholar 

  • Jensen, V.: The Azotobacter-flora of some Danish water courses. Dansk bot. Tidsskr. 52, 143–157 (1955).

    Google Scholar 

  • Kellenberger, E., A. Ryter and J. Séchaud: Electron microscope study of DNA-containing plasmas. J. biophys. biochem. Cytol. 4, 671 (1958).

    Google Scholar 

  • Löhnis, F., u. J. Hanzawa: Die Stellung von Azotobacter im System. Zbl. Bakt., II, Abt. 42, 1–8 (1914).

    Google Scholar 

  • Löhnis, F., and N. R. Smith: Studies upon the life-cycles of bacteria. II. Life history of Azotobacter. J. Agr. Res. 23, 401–432 (1923).

    Google Scholar 

  • Mayall, B. H., and C. F. Robinow: Observations with the electron microscope on the organization of the cortex of testing and germin2ting spores of B. megaterium. J. appl. Bact. 20, 333 (1957).

    Google Scholar 

  • Murray, R. G. E.: The internal structure of the cell. In: The Bacteria. Ed. by J. C. Gunsalus and R. Y. Stanier, Vol. I, pp. 35–96. New York and London: Academic Press 1960.

    Google Scholar 

  • Murray, R. G. E.: The structure of microbial cytoplasm and the development of membrane systems. In: Lectures on Theoretical and Applied Aspects of Modern Microbiology. 2, University of Maryland, College Park, Maryland 1960.

    Google Scholar 

  • Petersen, E. J.: Studies on the morphology and cytology of Azotobacter chroococcum. Royal Vet. Agr. Coll., Copenhagen. Yearbook 1961, pp. 115–155.

  • Robinow, C. F.: Morphology of bacterial spores, their development and germination. In: The Bacteria, Ed. by J. C. Gunsalus and R. Y. Stanier, Vol. I, pp. 207–248. New York and London: Academic Press 1960.

    Google Scholar 

  • Ryter, A., et E. Kellenberger: Étude au microscope électronique de plasmas contenant de l'acide desoxyribonucléique. Z. Naturforsch. 13 b, 597–605 (1958).

    Google Scholar 

  • Schlegel, H. G., G. Gottschalk and R. von Bartha: Formation and utilization of poly-β-hydroxybutyric acid by Knallgas-bacteria (Hydrogenomonas). Nature (Lond.) 191, 463–465 (1961).

    Google Scholar 

  • Shinohara, C., K. Fukushi and J. Suzuki: Mitochondria-like structures in ultrathin sections of Mycobacterium avium. J. Bact. 74, 415 (1957).

    Google Scholar 

  • Socolofsky, M. D., and O. Wyss: Cysts of Azotobacter. J. Bact. 81, 946–954 (1961).

    Google Scholar 

  • Tchan, Y. T.: Studies of N-fixing bacteria. IV. Taxonomy of genus Azotobacter (Beijerinck 1901). Proc. Linn. Soc. N.S.Wales 78, 85–89 (1953).

    Google Scholar 

  • Winogradsky, S.: Sur la morphologie et l'oecologie des Azotobacter. Ann. Inst. Pasteur 60, 351–400 (1938).

    Google Scholar 

  • Winogradsky, S.: Microbiologie du sol, p. 746–780. Paris: Masson et Cie. 1949.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tchan, Y.T., Birch-Andersen, A. & Jensen, H.L. The ultrastructure of vegetative cells and cysts of Azotobacter chroococcum. Archiv. Mikrobiol. 43, 50–66 (1962). https://doi.org/10.1007/BF00408395

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00408395

Keywords

Navigation