Abstract
Glucans from the fish pathogen Aeromonas hydrophila have been extracted andpurified by a method utilizing phenol/water followed by sodium deoxycholate rather than the traditional sodium hydroxide extraction. Presence of substantial amounts of these glucans was shown to be dependant on whether or not the substrate contained dextrose, a point which had import because of the low carbohydrate environment in which this species must survive and multiply. These glucans, produced in the log phase, were utilized during the later growth period.
The structures of the two purified glucans were examined by methylation analysis, periodate oxidation, and enzymatic degradation. The results indicated that A. hydrophila under low-carbohydrate growth conditions produced two similar but distinguishable α1→4 linked glucans substituted α1→6 by single monosaccharide residues or short chains to give an amylopectinglycogen type of polysaccharide.
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Shaw, D.H., Squires, M.J. Optional production and utilization of polysaccharide by Aeromonas hydrophila . Arch. Microbiol. 125, 83–87 (1980). https://doi.org/10.1007/BF00403202
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DOI: https://doi.org/10.1007/BF00403202