Abstract
The transition of blastospores to the mycelial phase in Candida albicans was induced after the blastospores were kept at 4°C for several hours and then transferred to a fresh medium prewarmed at 37°C. Glucan was the most abundant polymer in the wall in the two morphogenetic forms but the amount of chitin was higher in the mycelial form than in blastospores. Efficient protoplasting required reducing agents and proteases together with β-glucanases (zymolyase). Protein synthesis in regenerating protoplasts was initiated after about 30 min. Chitin synthetase, initially very low, was incorporated in important amounts into cell membranes mainly in a zymogenic state. After a few hours chitin was the most abundant polymer found in the aberrant wall of the regenerating protoplast.
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References
Anné, J., Eyssen, H. and De Somer, P. 1974. Formation and regeneration of Penicillium chrysogenum protoplasts. — Arch. Microbiol. 98: 159–166.
Benítez, T., Villa, T. G. and García Acha, I. 1975. Chemical and structural differences in mycelial and regeneration walls of Trichoderma viride. — Arch. Microbiol. 105: 277–282.
Cassone, A., Kerridge, D. and Gale, E. F. 1979. Ultrastructural changes in the cell wall of Candida albicans following cessation of growth and their possible relationship to the development of polyene resistance. — J. Gen. Microbiol. 110: 339–349.
Chattaway, F. W., Holmes, M. R. and Barlow, A. J. E. 1968. Cell wall composition of the mycelial and blastospore forms of Candida albicans. — J. Gen. Microbiol. 51: 367–376.
Demek, D. B. and Borgia, P. T. 1981. Changes in the rate of chitin-plus-chitosan synthesis accompany morphogenesis of Mucor racemosus. — J. Bacteriol. 146: 945–951.
Domer, J. E., Hamilton, J. G. and Harkin, J. C. 1967. Comparative study of the cell walls of the yeastlike and mycelial phases of Histoplasma capsulatum. — J. Bacteriol. 94: 466–474.
Dominguez, A., Varona, R. M., Villanueva, J. R. and Sentandreu, R. 1982. Mutants of Saccharomyces cerevisiae cell devision cycle defective in cytokinesis. Biosynthesis of the cell wall and morphology. — Antonie van Leeuwenhoek 48: 145–157.
Farkas, V. and Svoboda, A. 1980. Kinetics of β-glucan and chitin formation by cells and protoplasts of the yeast Saccharomyces cerevisiae. — Curr. Microbiol. 4: 99–103.
Garcia Mendoza, C. and Novaes Ledieu, M. 1968. Chitin in the new wall of regenerating protoplasts of Candida utilis. — Nature (London) 220: 1035.
Gibson, R. K. and Peberdy, J. F. 1972. Fine structure of protoplasts of Aspergillus nidulans. —J. Gen. Microbiol. 72: 529–538.
Kessler, G. and Nickerson, W. J. 1959. Glucomannan-protein complexes from cell walls of yeast. — J. Biol. Chem. 234: 2281–2285.
Lee, K. L., Buckley, H. R. and Campbell, C. 1975. An aminoacid liquid synthetic medium for development of mycelial and yeast forms of Candida albicans. — Sabouraudia 13: 148–153.
Manning, M. and Mitchell, T. G. 1980. Morphogenesis of Candida albicans and cytoplasmic proteins associated with differences in morphology, strain or temperature. — J. Bacteriol. 144: 258–273.
Maresca, B., Lambowitz, A. M., Kumar, V. B., Grant, G. A., Kobayashi, G. S. and Medoff, G. 1981. Role of cysteine in regulating morphogenesis and mitochondrial activity in the dimorphic fungus Histoplasma capsulatum. — Proc. Natl Acad. Sci. USA 78: 4596–4600.
Medoff, J., Jacobson, E. and Medoff, G. 1981. Regulation of dimorphism in Histoplasma capsulatum by cyclic adenosine 3′, 5′-monophosphate. — J. Bacteriol. 145: 1452–1455.
Nickerson, W. J. and Falcone, G. 1956. Identification of protein disulfide reductase as a cellular division enzyme in yeasts. — Science 124: 722–723.
Shepherd, M. G., Yin, C. Y., Ram, S. P. and Sullivan, P. A. 1980. Germ tube induction in Candida albicans. — Can. J. Microbiol. 26: 21–26.
Sundaram, S., Sullivan, P. A. and Shepherd, M. G. 1981. Changes in lipid composition during starvation and germ-tube formation in Candida albicans. — Exp. Mycol. 5: 140–147.
Torres-Bauzá, L. J. and Riggsby, W. S. 1980. Protoplasts from yeast and mycelial forms of Candida albicans. — J. Gen. Microbiol. 119: 341–349.
Trevelyan, W. E., Procter, D. P. and Harrison, J. S. 1950. Detection of sugars on paper chromatograms. — Nature (London) 166: 444–445.
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Elorza, M.V., Rico, H., Gozalbo, D. et al. Cell wall composition and protoplast regeneration in Candida albicans . Antonie van Leeuwenhoek 49, 457–469 (1983). https://doi.org/10.1007/BF00399324
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DOI: https://doi.org/10.1007/BF00399324