Abstract
The relationship between adenine-nucleotide levels and metabolism-dependent membrane potential was studied in cells of Nitellopsis obtusa. Effects of ADP and AMP in the presence of ATP on electrogenic pump activity were measured in the dark, using the continuous perfusion method. Both ADP and AMP acte as competitive inhibitors for ATP, the Ki value for either compound being about 0.4 mM. The role of ADP and AMP as regulating factors for the electrogenic pump was investigated under various metabolic conditions. Application of N2 gas in the dark caused a significant membrane depolarization amounting to 90 mV, but cytoplasmic streaming and membrane excitability were not affected. Under anoxia, the ATP level decreased from 1.6 to 0.5 mM; ADP increased but only slightly, and AMP increased greatly. However, the time course of changes in the adenine nucleotides was not concurrent with that of the membrane-potential changes, thus, the adenine-nucleotide level changes cannot fully account for the N2-elicited depolarization. Under light, although the membrane hyperpolarized, no significant changes in the adenine-nucleotide levels were observed. Therefore, the light-induced membrane hyperpolarization cannot be explained solely by changes in adenine-nucleotide levels.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- APW:
-
artificial pond water
- Em :
-
membrane potential
- Rm :
-
membrane resistance
References
Brown, S.O. (1938) Relation between light and the electric polarity of Chara. Plant Physiol. 13, 713–736
Colowick, S.P., Kalckar, H.M. (1943) The role of myokinase in transphosphorylation. I. The enzymatic phosphorylation of hexoses by adenyl pyrophosphate. J. Biol. Chem. 148, 117–126
Felle, H., Bentrup, F.W. (1977) A study of the primary effect of the uncoupler carbonyl cyanide m-chlorophenylhydrazone on membrane potential and conductance in Riccia fluitans. Biochim. Biophys. Acta 464, 179–187
Fujii, S., Shimmen, T., Tazawa, M. (1979) Effect of intracellular pH on the light-induced potential change and electrogenic activity in tonoplast-free cells of Chara australis. Plant Cell Physiol. 20, 1315–1328
Goller, M., Hampp, R., Ziegler, H. (1982) Regulation of the cytosolic adenylate ratio as determined by rapid fractionation of mesophyll protoplasts of oat. Planta 156, 255–263
Hampp, R., Goller, M., Ziegler, H. (1982) Adenylate levels, energy charge and phosphorylation potential during dark-light and light-dark transition in chloroplasts, mitochondria and cytosol of mesophyll protoplasts from Avena sativa L. Plant Physiol. 69, 448–455
Holm-Hansen, O., Karl, D.M. (1978) Biomass and adenylate energy charge determination in microbial cell extracts and environmental samples. Methods Enzymol. 57, 73–85
Keifer, D.W., Spanswick, R.M. (1979) Correlation on adenosine triphosphate level in Chara corallina with the activity of the electrogenic pump. Plant Physiol. 64, 165–168
Löppert, H. (1983) Energy coupling for membrane hyperpolarization in Lemna: respiration rate, ATP level and membrane potential at low oxygen concentration. Planta 159, 329–335
Mimura, T., Shimmen, T., Tazawa, M. (1982) Respiration-dependent membrane hyperpolarization in tonoplast-free cells of Nitella axilliformis. Plant Cell Physiol. 23, 1419–1425
Mimura, T., Shimmen, T., Tazawa, M. (1983) Dependence of the membrane potential on intracellular ATP concentration in tonoplast-free cells of Nitellopsis obtusa. Planta 157, 97–104
Nagai, R., Tazawa, M. (1962) Changes in resting potential and ion absorption induced by light in a single plant cell. Plant Cell Physiol. 3, 323–339
Saito, K., Senda, M. (1973) The light-dependent effect of external pH on the membrane potential of Nitella. Plant Cell Physiol. 14, 147–156
Shimmen, T. (1978) Dependency of cytoplasmic streaming on intracellular ATP and Mg2+ concentrations. Cell Struct. Funct. 3, 113–121
Shimmen, T., Kikuyama, M., Tazawa, M. (1976) Demonstration of two stable potential states of plasmalemma of Chara without tonoplast. J. Membr. Biol. 30, 249–270
Shimmen, T., Tazawa, M. (1977) Control of membrane potential and excitability of Chara cells with ATP and Mg2+. J. Membr. Biol. 37, 167–192
Shimmen, T., Tazawa, M. (1980a) Dependency of H+ efflux on ATP in cells of Chara australis. Plant Cell Physiol. 21, 1007–1013
Shimmen, T., Tazawa, M. (1980b) Intracellular chloride and potassium ions in relation to excitability of Chara membrane. J. Membr. Biol. 55, 223–232
Smith, P.T., Walker, N.A. (1981) Studies on the perfused plasmalemma of Chara corallina. I. Current-voltage curves: ATP and potassium dependence. J. Membr. Biol. 60, 223–236
Spanswick, R.M. (1981) Electrogenic ion pumps. Annu. Rev. Plant Physiol. 32, 267–289
Stitt, M., Lilley, McC. R., Heldt, W.H. (1982) Adenine nucleotide levels in the cytosol, chloroplasts and mitochondria of wheat leaf protoplasts. Plant Physiol. 70, 971–977
Takeuchi, Y., Kishimoto, U. (1983) Changes of adenine nucleotide levels in Chara internodes during metabolic inhibition. Plant Cell Physiol. 24, 1401–1409
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mimura, T., Shimmen, T. & Tazawa, M. Adenine-nucleotide levels and metabolism-dependent membrane potential in cells of Nitellopsis obtusa Groves. Planta 162, 77–84 (1984). https://doi.org/10.1007/BF00397424
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00397424