Abstract
The analytical model describing the steady state position of chloroplasts in dependence of fluence rate as well as the chloroplast response to single strong light pulses has been proposed. The model is based on the following assumptions: 1. Irradiation of the cell generates the state X in the cell membrane region, proportional to the local fluence rate. After switching on the light, the value of X increases exponentially with the time constant of about 3 min. The dark decay of X is also exponential with the same time constant. The level of X controls all kinds of chloroplast arrangements. 2. The state X generates two further states: Y 1 and Y 2, the first of them representing attraction forces for chloroplasts and the second representing repulsion forces. Empirical equations have been found for both Y states. The fluence rate response curve can be described with the use of functions Y 1 and Y 2. 3. The kinetic analysis requires the introduction of two additional functions Z in order to account for delays and time dispersion of the chloroplast movement in response to driving and resistance factors. The computer program for the proposed model was developed and the results of calculations were compared with experimental data (fluence rate response curve and pulse effects) with satisfactory agreement. Initially no attempt was made to ascribe any physical meaning to the postulated states. Some suggestions in this respect are mentioned in the discussion.
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References
Babuŝkin, L.N. (1955) Adaptation of the photosynthetic apparatus of chloroplasts to the light intensity. Dokl. Akad. Nauk SSSR. 102, 1215–1218
Bentrup, F.W. (1974) Lichtabhängige Membranpotentiale bei Pflanzen. Ber. Dtsch. Bot. Ges. 87, 515–528
Blatt, M.R., Briggs, W.R. (1980) Blue-light-induced cortical fiber reticulation concomitant with chloroplast aggregation in the alga Vaucheria sessilis. Planta 147, 355–362
Blatt, M.R., Weisenseel, M.H., Haupt, W. (1981) A light-dependent current associated with chloroplast aggregation in the alga Vaucheria sessilis. Planta 152, 513–526
Blatt, M.R., Wessells, N.K., Briggs, W.R. (1980) Actin and cortical fiber reticulation in the Siphonaceous alga Vaucheria sessilis. Planta 147, 363–375
Boscow, J.S., Feinleib, M.E. (1979) Phototactic response of Chlamydomonas to flashes of light. II. Response of individual cells. Photochem. Photobiol. 30, 499–505
Bottelier, H.P. (1934) Über den Einfluß äußerer Faktoren auf die Protoplasmaströmung in der Avena-Koleoptile. Rec. Trav. Bot. Neerl. 31, 474–582
Delbrück, M., Reichardt, W. (1956) System analysis for the light growth reactions of Phycomyces. In: Cellular mechanisms in differentiation and growth, pp. 3–44, Rudnick, D., ed., Princeton Univ. Press, Princeton
Feinleib, M.E. (1975) Phototactic response of Chlamydomonas to flashes of light. I. Response of cell populations. Photochem. Photobiol. 21, 351–354
Fischer-Arnold, G. (1963) Untersuchungen über die Chloroplastenbewegung bei Vaucheria sessilis. Protoplasma 56, 495–520
Gabryś-Mizera, H. (1976) Model consideration on the light conditions in noncylindrical plant cells. Photochem. Photobiol. 25, 453–461
Gabryś, H., Walczak, T. (1980) Photometric study of chloroplast photo-translocations in leaves of land plants. Acta Physiol. Plant. 2, 281–290
Gabryś, H., Walczak, T., Zurzycki, J. (1981) Chloroplast translocation induced by light pulses. Effects of single light pulses. Planta 152, 553–556
Haupt, W. (1959) Die Chloroplastendrehung bei Mougeotia. Über den quantitativen und qualitativen Lichtbedarf der Schwachlichtbewegung. Planta 53, 484–501
Haupt, W., Reif, G. (1978) Short-term reactions in phytochrome-regulation chloroplast orientation. Acta Protozool. 18, 145
Haupt, W., Thiele, R. (1961) Chloroplastenbewegung bei Mesotaenium. Planta 56, 388–401
Hellewell, S.B., Taylor, D.L. (1979) The contractile basis of ameboid movement. VI. The solation-contraction coupling hypothesis. J. Cell Biol. 83, 633–648
Inoue, Y., Shibata, K. (1973) Light-induced chloroplast rearrangements and their action spectra as measured by absorption spectroscopy. Planta 114, 341–358
Mayer, F. (1964) Lichtorientierte Chloroplasten-Verlagerung bei Selaginella martensii. Z. Bot. 52, 346–381
Schönbohm, E. (1963) Untersuchungen über die Starklictbbe-wegung des Mougeotia-Chloroplasten. Z. Bot. 51, 233–276
Schönbohm, E. (1972) Experiments on the mechanism of chloroplast movement and light oriented chloroplast arrangement. Acta Protozool. 11, 211–223
Schönbohm, E. (1975) Der Einfluß von Colchicin sowie von Cytochalasin B auf fädige Plasmastrukturen, auf die Verankerung der Chloroplasten sowie auf die orientierte Chloroplastenbewegung. Ber. Dtsch. Bot. Ges. 88, 211–224
Seitz, K. (1967) Wirkungsspektren für die Starklichtbewegung der Chloroplasten, die Photodinese und die lichtabhängige Viskositätsänderung bei Vallisneria spiralis ssp. torta. Z. Pflanzenphysiol. 56, 246–261
Seitz, K. (1972) Primary processes controlling the light induced movement of chloroplasts. Acta Protozool 11, 225–235
Virgin, H.I. (1951) The effect of light on the protoplasmic viscosity. Physiol. Plant 4, 255–357
Walczak, T., Gabryś, H. (1980) New type of photometer for measuring of transmission changes corresponding to chloroplast movements in leaves. Photosynthetica 14, 65–75
Zurzycka, A., Zurzycki, J. (1954) Studies on the phototactic movements of chloroplasts. II. Acta Soc. Bot. Polon. 23, 279–288
Zurzycki, J. (1962) The action spectrum for the light dependent movementts of chloroplasts in Lemna trisulca L Acta Soc. Bot. Polon. 31, 489–538
Zurzycki, J. (1965) The energy of chloroplast movements in Lemna trisulca L. Acta Soc. Bot. Polon. 34, 637–666
Zurzycki, J. (1967) Properties and localisation of the photoreceptor active and displacements od chloroplasts in Funaria hygrometrica. I. Action spectrum. Acta Soc. Bot. Polon. 36, 133–142
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Zurzycki, J., Walczak, T., Gabryś, H. et al. Chloroplast translocations in Lemna trisulca L. induced by continuous irradiation and by light pulses. Planta 157, 502–510 (1983). https://doi.org/10.1007/BF00396880
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DOI: https://doi.org/10.1007/BF00396880