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ATPase activity associated with isolated vacuoles of the crassulacean acid metabolism plant Kalanchoë daigremontiana

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Abstract

A technique is described that allows a relatively rapid and controlled isolation of vacuoles from leaves of the crassulacean acid metabolism (CAM) plant Kalanchoë daigremontiana. The method involves polybase-induced lysis of mesophyllcell protoplasts and isolation of vacuoles on a discontinuous density gradient. ATPase activity is associated with the isolated vacuoles and is not attributable to contamination by cytoplasmic constituents. It is suggested that this ATPase is responsible for the energization of malic-acid accumulation in the vacuole in CAM plants.

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Abbreviations

CAM:

crassulacean acid metabolism

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Author notes

  1. Ernest G. Uribe

    Present address: Department of Botany, Washington State University, 99164, Pullman, WA, USA

Authors and Affiliations

  1. Institut für Botanik, Technische Hochschule, Schnittspahnstrasse 3-5, D-6100, Darmstadt, Federal Republic of Germany

    J. Andrew C. Smith, Ernest G. Uribe, Erika Ball & Ulrich Lüttge

Authors
  1. J. Andrew C. Smith
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  2. Ernest G. Uribe
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  3. Erika Ball
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  4. Ulrich Lüttge
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Dedicated to Professor Dr. W. Simonis on the occasion of his 75th birthday

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Smith, J.A.C., Uribe, E.G., Ball, E. et al. ATPase activity associated with isolated vacuoles of the crassulacean acid metabolism plant Kalanchoë daigremontiana . Planta 162, 299–304 (1984). https://doi.org/10.1007/BF00396740

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  • DOI: https://doi.org/10.1007/BF00396740

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