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Differentiation of species in the Bacillus brevis group and the Bacillus aneurinolyticus group based on the electrophoretic whole-cell protein pattern

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Abstract

Ninety strains of eleven Bacillus species in the Bacillus brevis group and the Bacillus aneurinolyticus group were compared with the electrophoretic whole-cell protein pattern. The strains were separated into two clusters at the similarity of 55%. One cluster (cluster 1) was consisted of strains from the B. brevis group, and another cluster (cluster 2) was composed of strains from the B. aneurinolyticus group. The cluster 1 was separated into eight subclusters. Out of eight subclusters, seven subclusters contain strains from B. brevis, B. laterosporus, B. agri, B. reuszeri, B. choshinensis, B. formosus, and B. borstelensis. Another subcluster was further separated into two related clusters, which corresponded to B. centrosporus and B. parabrevis, and they were fused at the similarity of 76%. Cluster 2 was separated into two subclusters, which corresponded to B. aneurinolyticus and B. migulanus. The above eleven species showed characteristic patterns distinct from one another, and this correlated well with the published DNA relatedness data. The comparison of the electrophoretic whole-cell protein pattern proved to be useful for evaluation of taxonomic relationships among these taxa.

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Authors and Affiliations

  1. Research Laboratory, Higeta Shoyu Co., Ltd., 288, Choshi, Chiba, Japan

    Osamu Shida, Hiroaki Takagi & Kiyoshi Kadowaki

  2. Chugoku National Agricultural Experiment Station, Ministry of Agriculture, Forestry and Fisheries, 721, Fukuyama, Hiroshima, Japan

    Hiroshi Yano

  3. Department of Agricultural Chemistry, Faculty of Agriculture, Tokyo University of Agriculture, Setagaya-ku, 156, Tokyo, Japan

    Kazuo Komagata

Authors
  1. Osamu Shida
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  2. Hiroaki Takagi
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  3. Kiyoshi Kadowaki
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  4. Hiroshi Yano
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  5. Kazuo Komagata
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Shida, O., Takagi, H., Kadowaki, K. et al. Differentiation of species in the Bacillus brevis group and the Bacillus aneurinolyticus group based on the electrophoretic whole-cell protein pattern. Antonie van Leeuwenhoek 70, 31–39 (1996). https://doi.org/10.1007/BF00393567

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  • DOI: https://doi.org/10.1007/BF00393567

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