Abstract
Cotton fibres possess several β-glucanase activities which appear to be associated with the cell wall, but which can be partially solubilised in buffers. The main activity detected was that of an exo-(1→3)-β-d-glucanase (EC 3.2.1.58) but which also had the characteristics of a β-glucosidase (EC 3.2.1.21). Endo-(1→3)-β-d-glucanase activity (EC 3.2.1.39) and much lower levels of (1→4)-β-d-glucanase activity were also detected. The exo-(1→3)-β-glucanase showed a maximum late on (40 days post-anthesis) in the development of the fibres, whereas the endo-(1→3)-β-glucanase activity remained constant throughout fibre development. The β-glucanase complex associated with the cotton-fibre cell wall also functions as a transglucosylase introducing, inter alia, (1→6)-β-glucosyl linkages into the disaccharide cellobiose to give the trisaccharide 4-O-β-gentiobiosylglucose.
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Abbreviations
- CMC:
-
carboxymethylcellulose
- ONPG:
-
o-nitrophenyl-β-d-glucopyranoside
- TLC:
-
thin-layer chromatography
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Bucheli, P., Dürr, M., Buchala, A.J. et al. β-Glucanases in developing cotton (Gossypium hirsutum L.) fibres. Planta 166, 530–536 (1985). https://doi.org/10.1007/BF00391278
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DOI: https://doi.org/10.1007/BF00391278