Summary
-
1.
All enzymes tested: malate dehydrogenase (MDH), glutamate dehydrogenase (GDH), glucose-6-phosphate dehydrogenase (G-6-PDH), and 6-phosphogluconate dehydrogenase (6-P-GDH)_behaved rhythmically during a period of continuous white light interrupted by darkness. All showed approximately the same frequency of 12–15 h.
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2.
The enzymes of the tricarboxylic-acid cycle (MDH and GDH) are in phase with each other but are out of phase with those of the oxidative pentose-phosphate pathway (G-6-PDH and 6-P-GDH) which are in turn in phase with each other.
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3.
GDH appears to be activated by the addition of Hoagland's solution which leads to an overt rhythm 24 h prior to darkness. The rhythms of MDH, G-6-PDH and 6-P-GDH cannot be demonstrated prior to the onset of darkness due to an inhibition of the MDH and pentose-phosphate cycle enzymes by light.
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4.
The control of the frequency and phase of these rhythms are discussed in relation to a positive correlation of the rhythms in enzyme activity presented here and the rhythms of the pyridine nucleotides presented elsewhere.
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Abbreviations
- ATP:
-
adenosine triphosphate
- G-6-PDH:
-
glucose-6-phosphate dehydrogenase
- GDH:
-
glutamate dehydrogenase
- MDH:
-
malate dehydrogenase
- NAD:
-
nicotinamide adenine dinucleotide
- NADH:
-
reduced NAD
- NADP:
-
nicotinamide adenine dinucleotide phosphate
- NADPH:
-
reduced NADP
- OAA:
-
oxaloacetic acid
- 6-P-GDH:
-
6-phosphogluconate dehydrogenase
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III=Frosch, S., Wagner, E. (1973).
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Deitzer, G.F., Kempf, O., Fischer, S. et al. Endogenous rhythmicity and energy transduction. Planta 117, 29–41 (1974). https://doi.org/10.1007/BF00388676
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DOI: https://doi.org/10.1007/BF00388676