Abstract
Measurements with microelectrodes implanted into Samanea saman (Jacq.) Merrill leaf pulvini showed that membrane potentials were rhythmically sensitive to the application of sucrose. The magnitude of the electrical depolarizations induced by sucrose were dependent on the concentration of H+ in the medium, yet changes in [H+] alone did not greatly affect the potential. During sucrose-induced electrical depolarization, there was a slight increase in the pH of the bathing medium; both effects were abolished by high levels of K+, Na+ or Ca2+ in the medium. These observations indicate that H+ enter the cells by some cooperative action with sucrose. A model of H+-substrate cotransport is proposed in which a sugar carrier in the membrane is made more permeable by the attachment of a proton. The rhythmic nature of this proposed cotransport may be related to circadian leaf-movements in this plant.
Similar content being viewed by others
References
Anderson, W.P., Hendrix, D.L., Higinbotham, N.: Higher plant cell membrane resistance by a single intracellular electrode method. Plant Physiol. 53, 122–124 (1974)
Etherton, B.: Rapid changes in membrane potentials of oat coleoptile cells induced by amino acids and carbohydrates. (Abstr.) Plant Physiol. Ann. Suppl., p. 49 (1974)
Goldsmith, M.H.M., Fernández, H.R., Goldsmith, T.H.: Electrical properties of parenchymal cell membranes in the oat coleoptile. Planta (Berl.) 102, 302–323 (1972)
Higinbotham, N., Etherton, B., Foster, R.J.: Mineral ion contents and cell transmembrane electropotentials of pea and oat seedling tissue. Plant Physiol. 42, 37–46 (1967)
Jones, M.G.K., Novacky, A., Dropkin, V.H.: Transmembrane potentials of parenchyma cells and nematode-induced transfer cells. Protoplasma 85, 15–37 (1975)
Komor, E., Tanner, W.: The hexose-proton symport system of Chlorella vulgaris. Europ. J. Biochem. 44, 219–223 (1974)
Njus, D., Sulzman, F.M., Hastings, J.W.: Membrane model for the circadian clock. Nature 248, 116–120 (1974)
Racusen, R.H., Satter, R.L.: Rhythmic and phytochrome-regulated changes in transmembrane potential in Samanea pulvini. Nature 225, 408–410 (1975)
Satter, R.L., Galston, A.W.: Leaf movements: rosetta stone of plant behavior? BioScience 23, 407–416 (1973)
Satter, R.L., Geballe, G.T., Applewhite, P.B., Galston, A.W.: Potassium flux and leaf movement in Samanea saman. I. Rhythmic movement. J. Gen. Physiol. 64, 413–430 (1974)
Satter, R.L., Marinoff, P., Galston, A.W.: Phytochrome controlled nyctinasty in Albizzia julibrissin. II. Potassium flux as a basis for leaflet movement. Amer. J. Bot. 57, 916–926 (1970)
Satter, R.L., Schrempf, M., Chaudri, J., Galston, A.W.: Phytochrome and circadian clocks in Samanea: Rhythmic redistribution of potassium and chloride within the pulvinus during long dark periods. Plant Physiol., in press (1976)
Schultz, S.G., Curran, P.F.: Coupled transport of sodium and organic solutes. Physiol. Rev. 50, 637–718 (1970)
Simon, E., Satter, R.L., Galston, A.W.: Circadian rhythmicity in excised Samanea pulvini, I. Sucrose-white light interactions. Plant Physiol. 58, 417–420 (1976)
Slayman, C.L.: Proton pumping and generalized energetics of transport: A review. In: Membrane Transport in Plants, pp. 107–119, Zimmermann, U., Dainty, J., eds. New York: Springer 1974
Slayman, C.L., Slayman, C.W.: Depolarization of the plasmamembrane of Neurospora during active transport of glucose: Evidence for a proton-dependent cotransport system. Proc. Natl. Acad. Sci. USA 71, 1935–1939 (1974)
Spanswick, R.M.: Evidence for an electrogenic ion pump in Nitella translucens I. The effects of pH, K+, Na+, light and temperature on the membrane potential and resistance. Biochim. Biophys. Acta 288, 73–89 (1972)
Toriyama, H., Jaffe, M.J.: Migration of calcium and its role in the regulation of seismonasty in the motor cell of Mimosa pudica L. Plant Physiol. 49, 72–81 (1972)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Racusen, R.H., Galston, A.W. Electrical evidence for rhythmic changes in the cotransport of sucrose and hydrogen ions in Samanea pulvini. Planta 135, 57–62 (1977). https://doi.org/10.1007/BF00387976
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00387976