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Cytochemical localization of glycolate oxidase in microbodies of Klebsormidium

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Abstract

The filamentous green alga Klebsormidium flaccidum A.Br. was fixed with glutaraldehyde, incubated in a cytochemical medium designed to detect glycolate-oxidase activity, and prepared for electron microscopy. Heavy deposits of stain were observed in microbodies following incubation with either glycolate or L-lactate as substrate, but not after incubation with D-lactate or H2O. When Chlamydomanas reinhardi Dangeared cells were treated in the same way, their microbodies did not appear stained. The results establish that in Klebsormidium glycolate-oxidase occurs in microbodies (peroxisomes), as it does in angiosperms; also, they emphasize the dichotomy between those green algae which contain glycolate-oxidase and those, such as Chlamydomonas, which possess the mitochondrial enzyme glycolate dehydrogenase.

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Authors and Affiliations

  1. Department of Biological Sciences, Mount Holyoke College, 01075, South Hadley, MA, USA

    P. J. Gruber & S. E. Frederick

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  1. P. J. Gruber
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  2. S. E. Frederick
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Gruber, P.J., Frederick, S.E. Cytochemical localization of glycolate oxidase in microbodies of Klebsormidium . Planta 135, 45–49 (1977). https://doi.org/10.1007/BF00387974

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  • DOI: https://doi.org/10.1007/BF00387974

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