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Abscisic acid binding to subcellular fractions from leaves of Vicia faba

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Abstract

A centrifugation binding assay has been used to demonstrate the binding of [3H] (±) abscisic acid to membrane-rich fractions prepared from leaves of Vicia faba L. Kinetic analysis of this binding shows evidence of saturation of binding sites with increasing concentration of ligand. Scatchard analysis of these data yields a biphasic plot possibly indicating the presence of two types of binding sites. The dissocation constant for the high affinity site has been calculated to be 3.5×10-8 mol 1-1.

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References

  • Batt, S., Venis, M.A.: Separation and localisation of two classes of auxin binding in corn coleoptile membranes. Planta 130, 15–21 (1976)

    Google Scholar 

  • Batt, S., Wilkins, M.B., Venis, M.A.: Auxin binding to corn coleoptile membranes: kinetics and specificity. Planta 130, 7–13 (1976)

    Google Scholar 

  • Hertel, R., Thomson, K., Russo, V.E.A.: In vitro auxin binding to particulate cell fractions from corn coleoptiles. Planta 107, 325–340 (1972)

    Google Scholar 

  • Hodges, T.K., Leonard, R.T.: Purification of a plasma-membranebound adenosine triphosphatase from plant roots. In: Methods in Enzymology XXXII pp. 392–406. Fleischer, S., Packer, L., eds. New York: Academic Press 1974

    Google Scholar 

  • Horton, R.F., Bruce, K.R.: Inhibition by abscisic acid of the light and dark uptake of potassium by slices of Vicia faba leaves. Can. J. Bot. 50, 1915–1917 (1972)

    Google Scholar 

  • Meidner, H., Willmer, C.: Mechanics and Metabolism of Guard Cells. Curr. Adv. Plant Sci. 17, 1–15. July 1975

    Google Scholar 

  • Milborrow, B.V.: The Chemistry and Physiology of Abscisic Acid, Ann Rev. Plant Physiol. 25, 259–307 (1974)

    Google Scholar 

  • Ray, P.M., Dohrmann, U., Hertel, R.: Characterisation of naphthaleneacetic acid binding to receptor sites on cellular membranes of maize coleoptile tissue. Plant Physiol. 59, 357–364 (1977)

    Google Scholar 

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Authors and Affiliations

  1. Department of Biological Sciences, The Polytechnic, Wulfruna Street, WV1 ILY, Wolverhampton, U.K.

    T. J. Hocking, Jennifer Clapham & K. J. Cattell

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  1. T. J. Hocking
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  2. Jennifer Clapham
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  3. K. J. Cattell
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Hocking, T.J., Clapham, J. & Cattell, K.J. Abscisic acid binding to subcellular fractions from leaves of Vicia faba . Planta 138, 303–304 (1978). https://doi.org/10.1007/BF00386826

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  • DOI: https://doi.org/10.1007/BF00386826

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