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Transport of indoleacetic acid in intact roots of Phaseolus coccineus

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Summary

Indoleacetic acid (IAA)-5-3H (2×10-9) was applied to intact roots of Phaseolus coccineus seedlings at the apex or 2 cm above the apex, and the movement of IAA-3H and its metabolites traced by sectioning and chromatography. Basipetal movement of label occurred for 2 cm or less, declining exponentially, and the amount increased with time. Acropetal transport from above the apex showed quantitatively less movement of radioactivity. After a 6h treatment period a decline of label occurred in the first 0.5cm, below which there was a long distance movement of small amounts of label, mainly in IAA, towards the apex where the label concentrated by a factor of approximately 2. Short-distance basipetal movement consisted of about equal amounts of IAA and metabolites, and only metabolites were found in areas more basipetal than 2cm. Label from solutions of sucrose-14C and 3H2O followed the same general pattern of movement as label from IAA-3H, except that acropetal movement of water showed a steady decrease in the amount of label as the distance from the area of application increased. The short distance basipetal transport of label with the breakdown of IAA-3H indicates that the extent of basipetal movement was limited by catabolic processes. The acropetal pattern of IAA-3H movement with the concentration of the transported material close to the apex, is possibly the result of transport in the phloem.

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Authors and Affiliations

  1. Section of Genetics, Development and Physiology, Division of Biological Sciences, Cornell University, Ithaca, N.Y.

    P. J. Davies & E. K. Mitchell

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  1. P. J. Davies
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  2. E. K. Mitchell
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Davies, P.J., Mitchell, E.K. Transport of indoleacetic acid in intact roots of Phaseolus coccineus . Planta 105, 139–154 (1972). https://doi.org/10.1007/BF00385573

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