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Influence of photoperiod on seed development in the genetic line of peas G2 and its relation to changes in endogenous gibberellins measured by combined gas chromatography — Mass spectrometry

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Abstract

When apical senescence in the genetic line of peas G2 was prevented by short days fruit development was also found to be retarded. The levels of GA20 and GA29 in cotyledons and pods grown under long or short days were measured by gas chromatography — mass spectrometry multiple ion monitoring using extracts derivatised with deuterated trimethylsilyl groups as internal standards. The levels of GA20 but not GA29, were increased by short days. Conventional gas chromatography — mass spectrometry showed that relative to GA29 the levels of GA19, the other GA identified in G2 cotyledons, were also increased in short days. The levels of GA20 in the pods were highest during the main phase of pod growth early in fruit development.

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Abbreviations

GAn :

gibberellin An

GC/MS:

gas chromatography — mass spectrometry

MIM:

multiple ion monitoring

Me:

methyl ester

SIM:

single ion monitoring

TIC:

total ion current

TMS:

trimethylsilyl ether

TLC:

thin layer chromatography

TTLC:

instant thin layer chromatography

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  1. John Innes Institute, Colney Lane, NR4 7UH, Norwich, UK

    T. J. Ingram & G. Browning

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  1. T. J. Ingram
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  2. G. Browning
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Ingram, T.J., Browning, G. Influence of photoperiod on seed development in the genetic line of peas G2 and its relation to changes in endogenous gibberellins measured by combined gas chromatography — Mass spectrometry. Planta 146, 423–432 (1979). https://doi.org/10.1007/BF00380856

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