Summary
Two chromosomal mutations in yeast that result in oversecretion of the K1 killer toxin protein were examined. A recessive mutation in gene ski5 appears to lead to toxin oversecretion through a defect in a cell surface, PMSF-inhibited protease. A wild type killer strain degraded toxin following synthesis, and degradation could be partially prevented by addition of PMSF to the growth medium. The ski5 mutation caused an approximate ten fold oversecretion of toxin, similar to that seen in a PMSF-treated wild type culture, and no increased oversecretion in the presence of PMSF. The ski5 mutation caused oversecretion of other low molecular weight secreted proteins and appeared to oversecrete the α-factor pheromone, as judged by activity tests. The ski5 mutation was complemented by mutations in ski genes 1–4, and the mutant was not supersensitive to mating pheromones or K2 killer toxin.
We also examined killer strains with a mutation in the nuclear gene krel which results in a defective (1→6)-β-D-glucan cell wall receptor for killer toxin. Such strains oversecrete toxin into the growth medium, but also, unexpectedly, oversecrete most other secreted proteins. The defect in (1→6)-β-D-glucan in these mutants appears to perturb the partitioning of secreted proteins between the cell wall and the medium.
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References
Al-Aidroos K, Bussey H (1978) Can J Microbiol 24:228–237
Balton C (1982) In: Strathern JN, Jones EW, Broach JR (eds) The Molecular Biology of the Yeast Saccharomyces, Metabolism and Gene Expression. Cold Spring Harbor Laboratory, pp 335–360
Bostian KA, Jayachandran S, Tipper DJ (1983) Cell 32:169–180
Bostian KA, Hopper JE, Rogers DT, Tipper DJ (1980) Cell 19:403–414
Bruenn JA, Kane W (1978) J Virol 26:762–772
Bussey H (1981) Adv Microb Physiol 22:93–121
Bussey H, Sacks W, Galley D, Saville D (1982) Mol Cell Biol 2:346–354
Bussey H, Saville D, Greene D, Tipper DJ, Bostian KA (1983) Mol Cell Biol 3 (in press)
Bussey H, Saville D, Hutchins K, Palfree RGE (1979) J Bacteriol 140:888–892
Ciejek E, Thorner J (1979) Cell 18:623–635
Chan RK, Otte CA (1982a) Mol Cell Biol 2:11–20
Chan RK, Otte CA (1982b) Mol Cell Biol 2:21–29
Finkelstein DB, Strausberg S (1979) J Biol Chem 254:796–803
Hutchins K, Bussey H (1983) J Bacteriol 154:161–169
Julius D, Blair L, Brake A, Sprague G, Thorner J (1983) Cell 32:839–852
Kurjan J, Herskowitz I (1982) Cell 30:933–943
Laemmli UK (1970) Nature (London) 227:680–685
Novick P, Schekman R (1979) Proc Natl Acad Sci USA 76:1858–1862
Pugsley AP (1983) J Gen Microbiol 129:833–840
Rogers D, Bevan EA (1978) J Gen Microbiol 105:199–202
Sherman F, Fink GR, Lawrence CW (1971) Methods in yeast genetics. Cold Spring Harbor Laboratory, pp 5–6
Schekman R, Novick P (1982) In: Strathern JN, Jones EW, Broach JR (eds) The Molecular Biology of the Yeast Saccharomyces, Metabolism and Gene Expression. Cold Spring Harbor Laboratory, pp 361–393
Toh-e A, Guerry P, Wickner RB (1978) J Bacteriol 136:1002–1007
Toh-e A, Wickner RB (1980) Proc Natl Acad Sci USA 77:527–530
Wickner RB (1981) In: Strathern JN, Jones EW, Broach JR (eds) The Molecular Biology of the Yeast Saccharomyces, Life Cycle and Inheritance. Cold Spring Harbor Laboratory, pp 415–444
Woods DR, Bevan EA (1968) J Gen Microbiol 51:115–126
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Bussey, H., Steinmetz, O. & Saville, D. Protein secretion in yeast: Two chromosomal mutants that oversecrete killer toxin in Saccharomyces cerevisiae . Curr Genet 7, 449–456 (1983). https://doi.org/10.1007/BF00377610
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DOI: https://doi.org/10.1007/BF00377610