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Isolation and characterization of Urbain, a 20-hydroxyecdysone-inducible gene expressed during morphogenesis of Bombyx mori wing imaginal discs

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Abstract

In insects, wing imaginal discs respond to the steroid hormone 20-hydroxyecdysone by initiating morphogenesis leading to the formation of the adult flight appendages. In this work we analyse the expression of a Bombyx gene, referred to as Urbain, whose cDNA had been previously isolated from wing discs (Chareyre et al. 1993). Accumulation of the 1.8 kb transcript occurs concomitantly with the increase of 20-hydroxyecdysone titer at every stage examined during post-embryonic development. In vitro, its accumulation is delayed 6–9 h after exposure to 20-hydroxyecdysone. Studies in the presence of cycloheximide have established that Urbain is a secondary response gene. The sequence of the mRNA contains a putative open reading frame of 1656 nucleotides encoding a secreted hydrophilic protein, and we suggest that the Urbain gene product plays a role in cellular mechanisms involved in morphogenesis of the epithelium.

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Authors and Affiliations

  1. Centre de Génétique Moléculaire et Cellulaire-UMR 106, Université Lyon-1, 43 bd du 11 novembre 1918, F-69622, Villeurbanne Cedex, France

    Marie Thérèse Besson, Pascal Chareyre, Jocelyne Demont, Jacques Fourche & Georges Bosquet

  2. Institut de Biologic et de Chimie des Protéines, 7 passage du Vercors, F-69367, Lyon, Cedex 07, France

    Gilbert Deléage

  3. Laboratoire de Zoologie, UA 674, Université de Bourgogne, 6 bd Gabriel, F-21000, Dijon, France

    Brigitte Quennedey & Annie Courrent

Authors
  1. Marie Thérèse Besson
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  2. Pascal Chareyre
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  3. Gilbert Deléage
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  4. Jocelyne Demont
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  5. Brigitte Quennedey
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  6. Annie Courrent
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  7. Jacques Fourche
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  8. Georges Bosquet
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Besson, M.T., Chareyre, P., Deléage, G. et al. Isolation and characterization of Urbain, a 20-hydroxyecdysone-inducible gene expressed during morphogenesis of Bombyx mori wing imaginal discs. Roux's Arch Dev Biol 205, 333–343 (1996). https://doi.org/10.1007/BF00377213

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  • DOI: https://doi.org/10.1007/BF00377213

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