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Differential transport requirements of HLA and H-2 class I glycoproteins

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Abstract

Transport of human and mouse major histocompatibility complex class I glycoproteins has been examined in a transport deficient B-lymphoblastoid cell line × T-lymphoblastoid cell line (B-LCL × T-LCL) hybrid, 174 × CEM. T2 (T2). This cell line expresses no detectable endogenous HLA-B5 and reduced levels of HLA-A2 on its surface although these molecules are synthesized. In order to study this defect further, either HLA-Bw58 or HLA-B7 genomic clones were transfected into T2. Metabolic labeling and immune precipitation demonstrated biosynthesis of the Bw58 or 137 glycoprotein. However, like the endogenous HLA-B5 molecule, neither HLA-Bw58 nor HLA-B7 was expressed at the cell surface. The cloned genes were properly expressed on the surface of C1R, a control B-LCL. To determine if mouse class I alleles had the same transport requirements as the human class I glycoproteins, either mouse H-2D por H-2K b class I genes were introduced into T2. Surprisingly, the H-2 class I glycoproteins were transported to the cell surface normally. These data suggest a fundamental difference between human and mouse histocompatibility antigens in their requirements for intracellular transport.

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  1. Richard Murray

    Present address: DNAX Research Institute, 901 California Avenue, 94304, Palo Alto, CA, USA

Authors and Affiliations

  1. Department of Microbiology and Immunology, Duke University Medical Center, 27710, Durham, NC, USA

    Jeff Alexander, J. Alan Payne & Peter Cresswell

  2. Department of Microbiology and Immunology, University of North Carolina, 27599, Chapel Hill, NC, USA

    Richard Murray & Jeffrey A. Frelinger

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  1. Jeff Alexander
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  2. J. Alan Payne
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  3. Richard Murray
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  4. Jeffrey A. Frelinger
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  5. Peter Cresswell
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Alexander, J., Payne, J.A., Murray, R. et al. Differential transport requirements of HLA and H-2 class I glycoproteins. Immunogenetics 29, 380–388 (1989). https://doi.org/10.1007/BF00375866

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