Abstract
The intracellular pH (pHi) of the colonic tumour cell line HT29 cl.19A was studied by microspectrofluorometry using the pH-sensitive dye BCECF. Single cells within a confluent monolayer, grown in a polarized manner on permeable supports, were examined. An amiloride-sensitive Na+/H+ exchange and a stilbene-insensitive Cl− /HCO3 − exchange mechanism have been identified in the basolateral membrane. Removal of Na+ from the basolateral solution caused a decrease of pHi by 0.50±0.09 unit (n=4). Amiloride or Na+-free solution at the apical side had no effect on pHi. Cl− removal at the basolateral side led to an increase of pHi by 0.20±0.03 unit (n=4) whereas apical removal had no influence on pHi. This effect was independent of Na+ and was insensitive to 0.2 mM 4,4′-diisothiocyanatodihydrostilbene-2, 2′-disulphonic acid. A basolateral Cl−/ HCO3 − exchanger is the most likely explanation for this observation. The Na+/H+ exchange mechanism in the basolateral membrane is an acid extruder, whereas the C1−/HCO3 − exchanger is an acid loader. Both of these mechanisms are important for the maintenance of intracellular pH in HT29 cl.19A cells.
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Busche, R., Jeromin, A., v. Engelhardt, W. et al. Basolateral mechanisms of intracellular pH regulation in the colonic epithelial cell line HT29 clone 19A. Pflügers Arch 425, 219–224 (1993). https://doi.org/10.1007/BF00374170
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DOI: https://doi.org/10.1007/BF00374170