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RT1-Linked Ir and Is genes control the immune response to bovine insulin in the rat

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Abstract

The immune response to bovine or pork insulin (BI or PI, respectively) was studied in the rat using the in vitro insulin-induced lymphocyte-proliferation assay. Results indicated that 11 inbred rat strains were divided into categories of high and low responders. Two high responders, SDJ (RT1 u) and BN(RT1 n) inbred rat strains, appeared to recognize different antigenic determinant(s) on the insulin molecule. The results of linkage and segregation analyses in F1, F2, backcross, and partially congenic rats showed that the Ir gene (Ir-BI), which encodes the high responsiveness in the SDJ rats, is inherited associated with RT1 u, whereas the immune suppression gene (Is-BI), which encodes the low responsiveness in the WKA(RT1 k) rats, is inherited together with RT1 k. The Is-BI is the first major histocompatibility complex (MHC)-linked Is gene reported in the rat. The LEJ(RTI-A u B b) inbred rat strain showed a low response to BI, indicating that Ir-BI is closer to RTI-B/RTI-D region than to RTI-A.

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Abbreviations

BI:

bovine insulin

HEPES:

N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid

It:

immune response

Is:

immune suppression

MHC:

major histocompatibility complex

mol. wt.:

molecular weight

PI:

pork insulin

sc :

subcutaneously

SD:

standard deviation

SI:

stimulation index

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Authors and Affiliations

  1. Department of Pathology, Hokkaido University, School of Medicine, N15 W7, Sapporo, 060, Japan

    Tetsuo Inomata,  Takashi Natori, Yasuyuki Fujimoto, Shinya Tsutimoto & Miki Aizawa

  2. Department of Pediatric Dentistry, Hokkaido University, School of Dentistry, Sapporo, Japan

    Tetsuo Inomata & Kiyoshi Oikawa

Authors
  1. Tetsuo Inomata
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  2. Takashi Natori
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  3. Yasuyuki Fujimoto
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  4. Shinya Tsutimoto
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  5. Kiyoshi Oikawa
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  6. Miki Aizawa
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Inomata, T., Natori, T., Fujimoto, Y. et al. RT1-Linked Ir and Is genes control the immune response to bovine insulin in the rat. Immunogenetics 18, 373–385 (1983). https://doi.org/10.1007/BF00372470

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  • DOI: https://doi.org/10.1007/BF00372470

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