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An antiviral T-Cell clone defines a functional supertypic specificity shared by different HLA-DR molecules from DR2-short, DRw11, and DRwl3 Haplotypes

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Abstract

An influenza virus-specific HLA class IIrestricted human T4+ clone (Ij) allows us to define a new functional supertypic HLA class II specificity shared by three different haplotypes. Influenza A virus-infected antigen-presenting cells of these three haplotypes, HLA-DR2 short, DRw11, and DRw13, are able to stimulate Ij cells. The same precise viral specificity is seen in all three cases. Proliferation inhibition experiments using HLA-specific monoclonal antibodies demonstrate that HLA-DR products are involved in all cases. However, according to the DR specificity of the antigen-presenting cell, differential blockings by a series of DR-specific monoclonal antibodies suggest that the functional epitope is shared by different HLA DR molecules. This is confirmed by two-dimensional gel analysis of the HLA DRβ chains expressed in the three haplotypes.

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Abbreviations

APC:

antigen-presenting cell

EBV:

Epstein-Barr virus

HA:

hemagglutinin

HLA:

human leukocyte antigens

IL-2:

interleukin-2

MHC:

major histocompatibility complex

MLR:

mixed lymphocyte reaction

mAb:

monoclonal antibody

PAGE:

polyacrylamide gel electrophoresis

PBM:

peripheral blood mononuclear cells

% RR:

relative response percent

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Authors and Affiliations

  1. INSERM U152, CNRS UA, 620, Immunologie et Virologie des Tumeurs, Hôpital Cochin, 27, rue du faubourg Saint Jacques, F-75014, Paris, France

    Dominique Zeliszewski, Ghislaine Sterkers, Jeanine Choppin & Jean Paul Levy

  2. Centre de Transfusion Régional Lyon, 1, 3, rue du Vercors, F-69007, Lyon, France

    Catherine Freidel, Lucette Gebuhrer & Hervé Betuel

Authors
  1. Dominique Zeliszewski
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  2. Ghislaine Sterkers
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  3. Jeanine Choppin
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  4. Catherine Freidel
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  5. Lucette Gebuhrer
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  6. Hervé Betuel
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  7. Jean Paul Levy
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Zeliszewski, D., Sterkers, G., Choppin, J. et al. An antiviral T-Cell clone defines a functional supertypic specificity shared by different HLA-DR molecules from DR2-short, DRw11, and DRwl3 Haplotypes. Immunogenetics 25, 84–90 (1987). https://doi.org/10.1007/BF00364272

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  • DOI: https://doi.org/10.1007/BF00364272

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