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Different size of the product of the 14S light chain mRNA translated in vitro and in amphibian oocytes

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Abstract

When purified 14S mRNA for light chain of immunoglobulin is translated in a reticulocyte lysate and in Xenopus oocytes, two major differences are observed: (1) In the lysate 14S RNA competes efficiently with endogenous mRNA whereas in the oocyte it is translated without reducing the synthesis of endogenous proteins. (2) The translation product of 14S light chain mRNA in the lysate is a protein about 20 amino acids longer than light chain whereas in the oocyte it is a chain of the exact size of authentic secreted light chain. This difference can be explained if 14S mRNA codes for a precursor protein, which is not cleaved in the lysate but can be efficiently converted into light chain in the oocytes.

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References

  1. Mach, B., Faust, C. F., and Vassalli, P., Proc. Nat. Acad. Sci. U. S. 70, 451 (1973).

    Google Scholar 

  2. Faust, C. F., Diggelmann, H., and Mach, B., Biochemistry (Febr. 1973).

  3. Milstein, C., Brownlee, G. G., Harrison, T. M., and Matthews, M. B., Nature New Biol. 239, 117 (1972).

    Google Scholar 

  4. Gurdon, J., Lane, C., Woodland, H., and Marbaix, G., Nature 233, 177 (1971).

    Google Scholar 

  5. Moar, V. A., Gurdon, J. B., Lane, C. D., and Marbaix, G., J. Mol. Biol. 61, 93 (1971).

    Google Scholar 

  6. Berns, A. J. M., van Kraaikamp, M., Bloemendal, H., and Lane, C. D., Proc. Nat. Acad. Sci. U. S. 69, 1606 (1972).

    Google Scholar 

  7. Stevens, R. H. and Williamson, A. R., Nature 239, 143 (1972).

    Google Scholar 

  8. Lingrel, J. B., in: Methods in Molecular Biology, (Ed. J. A. Last and A. I. Laskin), M. Dekker, New York, Vol. 2, p. 231 (1972).

    Google Scholar 

  9. Gurdon, J. B., J Embryol. Exp. Morphol. 20, 401 (1968).

    Google Scholar 

  10. Mechler, B. and Mach, B., Europ. J. Biochem. 21, 552 (1971).

    Google Scholar 

  11. Laemmli, U. K., Nature 227, 680 (1970).

    Google Scholar 

  12. Dumont, J. N., J. of Morphology 136, 153 (1972).

    Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, University of Geneva, Switzerland

    B. Mach, C. F. Faust & P. Vassalli

  2. Department of Animal Biology, University of Geneva, Switzerland

    D. Rungger

Authors
  1. B. Mach
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  2. C. F. Faust
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  3. P. Vassalli
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  4. D. Rungger
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Mach, B., Faust, C.F., Vassalli, P. et al. Different size of the product of the 14S light chain mRNA translated in vitro and in amphibian oocytes. Molecular Biology Reports 1, 3–6 (1973). https://doi.org/10.1007/BF00357398

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  • DOI: https://doi.org/10.1007/BF00357398

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