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Nucleotide sequence relationships of double-stranded RNAs in flax rust, Melampsora lini

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Abstract

Flax rust, Melampsora lini strain SP6, contains 11 double-stranded (ds) RNA molecules with a total length of about 25 kbp. The dsRNAs are inherited in three genetic units: the L unit comprising a single 5.2 kbp dsRNA and contained within a 40-nm virus-like particle, and the A and B units each consisting of five dsRNAs (A1–A5, and B1–B5) ranging in size from 1.2 to 2.7 kbp. This paper reports the isolation of a cDNA library representing 10 of the 11 dsRNAs. By nucleic-acid hybridization techniques it has been shown that all ten sequences are unique showing no detectable cross-hybridization with any other dsRNA present in the rust. A near fulllength sequence of 1932 bp of the B3 dsRNA is reported and contains several open reading frames, the largest of which comprises most of the molecule.

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Author notes

  1. M. J. Dickinson

    Present address: University of Nottingham, Sutton Bonington Campus, 12 5RD, Loughborough, LE, UK

Authors and Affiliations

  1. Division of Plant Industry, CSIRO, GPO Box 1600, 2601, Canberra, ACT, Australia

    M. J. Dickinson, R. Zhang & A. Pryor

Authors
  1. M. J. Dickinson
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  2. R. Zhang
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  3. A. Pryor
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Communicated by O. C. Yoder

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Dickinson, M.J., Zhang, R. & Pryor, A. Nucleotide sequence relationships of double-stranded RNAs in flax rust, Melampsora lini . Curr Genet 24, 428–432 (1993). https://doi.org/10.1007/BF00351852

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  • DOI: https://doi.org/10.1007/BF00351852

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