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Modification of shell formation in the giant clam Tridacna gigas at elevated nutrient levels in sea water

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Abstract

The effect on shell formation of Tridacna gigas by sea water supplemented for 3 mo with ammonium (5, 10 μM, N) and phosphate (2, 5, 10 μM, P), separately or in combination, was examined. Exposure to N and N+P significantly enhanced shell-extension rates, but significantly reduced shell weights at equivalent size. Scanning-electron microscopy further revealed structural alterations in the outer shell layer, such as misshapen aragonite crystals, irregular crossed-lamellar orientation, and relatively porous shell microstructure. These observations are consistent with results of X-ray diffractometry on the shells which show distinct shifts in the positions of reflections from the (012) and (200) crystal planes relative to the control, indicating changes in crystal lattice parameters following addition of nutrients.

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Literature cited

  • Belda, C. A., Lucas, S., Yellowlees, D. (1993). Nutrient limitation in the giant clam-zooxanthellae symbiosis: effects of nutrient supplements on growth of the symbiotic partners. Mar. Biol. (in press)

  • Burton, E. A., Walter, L. M. (1990). The role of pH in phosphate inhibition of calcite and aragonite precipitation rates in sea water. Geochim. cosmochim. Acta 54: 797–808

    Google Scholar 

  • Crossland, C. J. (1983). Dissolved nutrients in coral reef waters. In: Barnes, D. J. (ed.) Perspectives in coral reefs. Australian Institute of Marine Science, Australia, p. 56–68

    Google Scholar 

  • Crossland, C. J., Barnes, D. J. (1983). Dissolved nutrients and organic particulates in water flowing over coral reefs at Lizard Island. Aust. J. mar. Freshwat. Res. 34: 835–844

    Google Scholar 

  • Hughes, T. P. (1989). Community structure and diversity of coral reefs: the role of history. Ecology 70: 275–279

    Google Scholar 

  • Kinsey, D. W. (1988). Coral reef system response to some natural and anthropogenic stresses. Galaxea 7: 113–128

    Google Scholar 

  • Kinsey, D. W. (1991). Water quality and its effects of reef ecology. In: Yellowlees, D. (ed.) Land use patterns and nutrient loading of the Great Barrier Reef Region. James Cook University Press, Australia, p. 192–196

    Google Scholar 

  • Kinsey, D. W., Davies, P. J. (1979). Effects of elevated nitrogen and phosphorus on coral-reef growth. Limnol. Oceanogr. 24: 935–940

    Google Scholar 

  • Liddicoat, M. I., Tibbits, S., Butler, E. I. (1975). The determination of ammonia in sea water. Limnol. Oceanogr. 20: 131–132

    Google Scholar 

  • Lutz R. A., Rhoads, D. C. (1980). Growth patterns within the molluscan shell: an overview. In: Rhoads, D. C., Lutz, R. A. (eds.) Skeletal growth of aquatic organisms: biological records of environmental change. Plenum Press, New York, p. 203–254

    Google Scholar 

  • Moir, B. G. (1990). Comparative studies of “fresh” and “aged” Tridacna gigas shells: preliminary investigations of a reported technique for pretreatment of tool material. J. archaeol. Sci. 17: 329–345

    Google Scholar 

  • Mucci, A., Morse, J. W. (1983). The incorporation of Mg2+ and Sr2+ into calcite overgrowths: influences of growth rate and solution composition. Geochim. cosmochim. Acta 47: 217–233

    Google Scholar 

  • Packard, G. C., Boardman, T. J. (1988). The misuse of ratios, indices, and percentages in ecophysiological research. Physiol. Zoöl. 61: 1–9

    Google Scholar 

  • Rasmussen, C. (1989) The use of strontium as an indicator of anthropogenically-altered environmental parameters. Proc. 6th int. coral Reef Symp. 2: 325–339 [Choat, J. H., et al. (eds.) Sixth International Coral Reef Symposium Executive Committee, Townsville]

  • Reddy, M. M. (1977). Crystallization of calcium carbonate in the presence of trace concentrations of phosphorus-containing anions. I. Inhibition by phosphate and glycerophosphate ions at pH 8.8 and 25°C. J. Crystal Growth 41: 287–295

    Google Scholar 

  • Rosewater, J. (1965). The family Tridacnidae in the Indo-Pacific. Indo-Pacif. Mollusca 1: 347–359

    Google Scholar 

  • Sands, D. E. (1969). Introduction to crystallography. W.A. Benjamin, Inc., New York

    Google Scholar 

  • Shapiro, S. S., Francia, R. S. (1972). An approximate analysis of variance test for normality. J. Am. statist. Ass. 67: 215–216

    Google Scholar 

  • Shapiro, S. S., Wilk, M. B. (1965). An analysis of variance test for normality (complete samples). Biometrika 52: 591–611

    Google Scholar 

  • Simkiss, K. (1964). Phosphates as crystal poisons of calcification. Biol. Rev. 39: 487–505

    Google Scholar 

  • Simkiss, K., Wilbur, K. M. (1989). Biomineralization: cell biology and mineral deposition. Academic Press, Inc., California

    Google Scholar 

  • Statistix 3.1. (1990). An interactive statistical analysis for microcomputers. Analytical Software, St Paul, Minnesota

  • Strickland, J. D. H., Parsons, T. R. (1972). A practical handbook of sea-water analysis. 2nd edn. Bull. Fish. Res. Bd Can. 167: 1–310

    Google Scholar 

  • Taylor, J. D., Kennedy, W. J., Hall, A. (1973). The shell structure and mineralogy of the Bivalvia: II. Lucinacea — Clavagellacea, conclusions. Bull. Br. Mus. nat. Hist. (Zool.) 22: 253–294

    Google Scholar 

  • Underwood, A. J. (1981). Techniques of analysis of variance in experimental ecology and marine biology. Oceanogr. mar. Biol. A. Rev. 19: 513–605

    Google Scholar 

  • Winer, B. J. (1971). Statistical principles in experimental design. McGraw-Hill, Tokyo

    Google Scholar 

  • Woodley, J. D., Chornesky, E. A., Clifford, P. A., Jackson, J. B. C., Kaufman, L. S., Knowlton, N., Lang, J. C., Pearson, M. P., Porter, J. W., Rooney, M. C., Rylaarsdam, K. W., Tunnicliffe, V. J., Wahle, C. M., Wulff, J. L., Curtis, A. S. G., Dallmeyer, M. D., Jupp, B. P., Koehl, M. A. R., Neigel, J., Sides, E. M. (1981). Hurricane Allen's impact on Jamaican coral reefs. Science, N. Y. 214: 749–755

    Google Scholar 

  • Yonge, C. M. (1975). Giant clams. Am. Scient. 232: 96–105

    Google Scholar 

  • Zar, J. H. (1984). Biostatistical analysis. 2nt edn. Prentice-Hall, Englewood Cliffs, N. J.

    Google Scholar 

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Communicated by G. F. Humphrey, Sydney

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Belda, C.A., Cuff, C. & Yellowlees, D. Modification of shell formation in the giant clam Tridacna gigas at elevated nutrient levels in sea water. Marine Biology 117, 251–257 (1993). https://doi.org/10.1007/BF00345670

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