Abstract
Using an indirect hemagglutination assay we tested sera from 94 healthy normal White Leghorn (NWL) and 117 Obese strain (OS) chickens with spontaneous autoimmune thyroiditis for the presence of “natural” antibodies against major histocompatibility complex (MHC) antigens expressed on red blood cells (RBC). In both groups older animals had a significantly increased frequency of such antibodies, but the titer was age-independent. OS chickens showed almost the same frequency of natural antibodies as NWL, and a comparison between OS birds with high antithyroglobulin autoantibody (Tg-AAb) titers and those with low titers also did not reveal a significant difference. We conclude that the occurrence of natural antibodies has no relation to Tg-AAb. The specificity of natural antibodies for MHC-encoded antigens was investigated in indirect immunofluorescence tests including absorption experiments with RBC and white blood cells (WBC). This analysis revealed that of 14 MHC-specific sera 13 were reacting with the B-G antigen, which is present on RBC only. One serum reacted with the B-F antigen, expressed on all somatic cells, i. e., both on RBC and WBC.
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Albini, B. and Wick, G.: Proportional increase of bursa-derived cells in chickens of the Obese strain. Nature 249: 653–654, 1974
Briles, W. E., Bumstead, N., Ewert, D. L., Gogusev, J., Hála, K., Koch, C., Longenecker, B. M., Nordskog, A. W., Pink, J. R. L., Schierman, L. W., Simonsen, M., Toivanen, A., Toivanen, P., Vainio, O., and Wick, G.: Nomenclature for the chicken major histocompatibility (B) complex. Immunogenetics 15: 441–447, 1982
Hála, K.: The major histocompatibility system of the chicken. In D. Götze (ed.): The Major Histocompatibility System in Man and Animals, pp. 291–312, Springer-Verlag, Berlin-Heidelberg-New York, 1977
Hála, K., Vilhelmová, M., and Hartmanová, J.: Probable crossing-over in the B blood group system of chickens. Immunogenetics 3: 97–103, 1976
Hála, K., Vilhelmová, M., and Hartmanová, J.: The structure of the major histocompatibility complex of the chicken. In A.A. Benedict (ed.): Avian Immunology, pp. 227–232, Plenum Press, New York, London, 1977
Hála, K., Plachý, J., and Schulmannová, J.: Role of the B-G-region antigen in the humoral immune response to the B-F-region antigen of chicken MHC. Immunogenetics 14: 393–401, 1981
Havele, C., Wegmann, T. G., and Longenecker, B. M.: Tolerance and autoimmunity to erythroid differentiation (B-G) major histocompatibility complex alloantigen of the chicken. J. Exp. Med. 156: 321–336, 1982
Hirata, A. A. and Terasaki, P. I.: Cross-reactions between streptococcal M proteins and human transplantation antigens. Science 168: 1095–1096, 1970
Iványi, P., van Mourik, P., Breuning, M., and Melief, C. J. M.: Anti-H-2 antibodies induced by syngeneic immunization. Immunogenetics 10: 319–332, 1980
Iványi, P., van Mourik, P., Breuning, M., Kruisbeek, A. M., and Kröse, C. J. M.: Natural H-2-specific antibodies in sera of aged mice. Immunogenetics 15: 95–102, 1982
Khoury, E. L., Bottazzo, G. F., de Carvalho, L., Wick, G., and Roitt, I. M.: Predisposition to organ-specific autoimmunity in Obese strain (OS) chickens: reactivity to thyroid, gastric, adrenal and pancreatic cytoplasmic antigens. Clin. Exp. Immunol. 49: 273–282, 1982
Longenecker, B. M. and Mosmann, T. R.: “Natural” antibodies to chicken MHC antigens are present in mice, rats, humans, alligators and allogeneic chickens. Immunogenetics 11: 293–302, 1980
Longenecker, B. M. and Mosmann, T. R.: Restricted expression of an MHC alloantigen in cells of the erythroid series: a specific marker for erythroid differentiation. J. Supramol. Struct. 13: 395–400, 1981a
Longenecker, B. M. and Mosmann, T. R.: Structure and properties of the major histocompatibility complex of the chicken. Speculations on the advantages and evolution of polymorphism. Immunogenetics 13: 1–23, 1981b
Longenecker, B. M., Mosmann, T. R., and Shiozawa, C.: A strong, preferential response of mice to polymorphic antigenic determinants of the chicken MHC, analyzed with mouse hybridoma (monoclonal) antibodies. Immunogenetics 9: 137–147, 1979
Makinodan, T. and Kay M. M. B.: Age influence on the immune system. Adv. Immunol. 29: 287–330, 1980
Miller, M. M., Goto, R., and Clark, S. D.: Structural characterization of developmentally expressed antigenic markers on chicken erythrocytes using monoclonal antibodies. Dev. Biol. 94: 400–414, 1982
Pink, J. R. L., Droege, W., Hála, K., Miggiano, V. C., and Ziegler, A.: A three-locus model for the chicken major histocompatibility complex. Immunogenetics 5: 203–216, 1977
Radl, J., Sepers, J. M., Skvaril, F., Morell, A., and Hijmans, W.: Immunoglobulin patterns in humans over 95 years of age. Clin. Exp. Immunol. 22: 84–90, 1975
Richter, E., Sundick, R., and Wick, G.: Heteroagglutinins and spontaneous rosette-forming cells in Obese-strain (OS) chickens. Z. Immunitätsforsch. Immunbiol. 149: 61–68, 1975
Simonsen, M., Crone, M., Koch, C., and Hála, K.: The MHC haplotypes of the chicken. Immunogenetics 16: 513–532, 1982
Wick, G.: The effect of bursectomy, thymectomy and X-irradiation on the incidence of precipitating liver and kidney autoantibodies in chickens of the Obese strain (OS). Clin. Exp. Immunol. 7: 187–199, 1970
Wick, G., Boyd, R., Hála, K., de Carvalho, L., Kofler, R., Müller, P.-U., and Cole, R. K.: The Obese strain (OS) of chickens with spontaneous autoimmune thyroiditis: Review of recent data. Curr. Top. Microbiol. Immunol. 91: 109–128, 1981
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Neu, N., Hála, K. & Wick, G. “Natural” chicken antibodies to red blood cells are mainly directed against the B-G antigen, and their occurrence is independent of spontaneous autoimmune thyroiditis. Immunogenetics 19, 269–277 (1984). https://doi.org/10.1007/BF00345400
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DOI: https://doi.org/10.1007/BF00345400