Summary
Cell fusion was used to obtain hybrid plants of rice (Oryza sativa L.) and four wild Oryza species, O. officinalis, O. eichingeri, O. brachyantha, and O. perrieri, to incorporate useful traits of the latter species into rice. A total of 250 mature hybrid plants were obtained by electrofusion and nurse culture methods. The hybrid nature of these plants was confirmed by karyotypic, morphological, and isozyme analysis. The hybrids of O. sativa+O. eichingeri, O. sativa+O. officinalis and O. sativa+O. perrieri produced viable pollen and the O. sativa+O. eichingeri hybrid has produced a progeny plant. Our study demonstrates the use of cell fusion for future rice improvement.
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References
Abdullah R, Cocking EC, Thompson JA (1986) Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio Technol 4:1087–1090
Bates GW, Hasenkampf CA, Contolini CL, Piastuch WC (1987) Asymmetric hybridization in Nicotiana by fusion of irradiated protoplasts. Theor Appl Genet 74:718–726
Cocking EC (1985) Somatic hybridization: Implications for agriculture. In: Zaitlin M, Day P, Hollaender A (eds) Biotechnology in plant science. Academic Press, New York, pp 101–113
Dudits D, Maroy E, Praznovszky T, Olah Z, Gyorgyey J, Cella R (1987) Transfer of resistance traits from carrot into tobacco by asymmetric somatic hybridization: Regeneration of fertile plants. Proc Natl Acad Sci USA 84:8434–8438
Fujimura T, Sakurai M, Akagi H, Negishi T, Hirose A (1985) Regeneration of rice plants from protoplasts. Plant Tissue Culture Lett 2:74–75
Kumashiro T, Kubo T (1986) Stability of agronomic traits in cytoplasmic male sterile tobacco obtained by protoplast fusion. Jpn J Breed 36:284–290
Kyozuka J, Hayashi Y, Shimamoto K (1987) High frequency plant regeneration from rice protoplasts by novel nurse culture methods. Mol Gen Genet 206:408–413
Morinaga T (1940) Cytogenetical studies on Oryza sativa L., 5. The cytogenetics of F1 hybrid of O. sativa L. and O. minuta Presl. Jpn J Bot 11:1–16
Morishima H (1984) Wild plant and domestication. In: Tsunoda S, Takahashi N (eds) Biology of rice. Jpn Sci Soc, Tokyo, Elsevier Amsterdam, pp 3–30
Nezu M, Katayama T, Kihara H (1960) genetic study of the genus Oryza. I. Crossability and chromosomal affinity among 17 species. Seiken Ziho 11:1–11
Ohira K, Ojima K, Fujiwara A (1973) Studies on the nutrition of rice cell culture 1. A simple, defined medium for rapid growth in suspension culture. Plant Cell Physiol 14:1113–1121
Ozias-Akins P, Ferl RJ, Vasil IK (1986) Somatic hybridization in the gramineae: Pennisetum americanum (L) K. Schum. (pearl millet)+Panicum maximum Jacq. (Guinea grass). Mol Gen Genet 203:365–370
Pelletier G, Primard C, Vedel F, Chetrit P, Remy R, Rouselle P, Renard M (1983) Intergeneric cytoplasmic hybridization in Cruciferae. Mol Gen Genet 191:244–250
Schieder O (1982) Somatic hybridization: A new method for plant improvement. In: Vasil IK, Scowcroft WR, Frey KJ (eds) Plant improvement and somatic cell genetics. Academic Press, New York, pp 239–253
Schieder O, Vasil IK (1980) Protoplast fusion and somatic hybridization. Int Rev Cytol [Suppl] 11B:21–46
Shinjo C (1984) Cytoplasmic male sterility and fertility restoration in rice having genome A. In: Tsunoda S, Takahashi N (eds) Biology of rice. Jpn Sci Soc, Tokyo Elsevier Amsterdam, pp 321–338
Swaminathan MS (1986) Plant research and world agriculture. Plant Mol Biol Rep 4:1–17
Tabaeizadeh Z, Ferl RJ, Vasil IK (1986) Somatic hybridization in the Gramineae. Saccharum officinarum L. (Sugarcane)+ Pennisetum americanum (L.) K. Shum. (Pearl millet). Proc Natl Acad Sci USA 83:5616–5619
Tateoka T (1957) Miscellaneous papers on the phylogeny of Poaceae (10). Proposition of new phylogenic system of Poaceae. J Jpn Bot 32:275–287
Terada R, Kyozuka J, Nishibayashi S, Shimamoto K (1987) Plantlet regeneration from somatic hybrids of rice (Oryza sativa L.) and barnyard grass (Eschinochloa oryzicola Vasing). Mol Gen Genet 210:39–43
Toriyama K, Hinata K, Sasaki T (1986) Haploid and diploid plant regeneration from protoplasts of anther callus in rice. Theor Appl Genet 73:16–19
Vallejos CE (1983) Enzyme activity staining. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, part A. Elsevier, Amsterdam, pp 469–516
Yamada Y, Yang TQ, Tang DT (1986) Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.). Plant Cell Rep 4:85–88
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Hayashi, Y., Kyozuka, J. & Shimamoto, K. Hybrids of rice (Oryza sativa L.) and wild Oryza species obtained by cell fusion. Mol Gen Genet 214, 6–10 (1988). https://doi.org/10.1007/BF00340171
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DOI: https://doi.org/10.1007/BF00340171