Skip to main content
Log in

Nitrate alters the flavonoid profile and nodulation in pea (Pisum sativum L.)

  • Original Paper
  • Published:
Biology and Fertility of Soils Aims and scope Submit manuscript

Abstract

A rhizosphere application of NO sup-inf3 and/or naringenin affected the Pisum sativum — Rhizobium leguminosarum biovar viciae symbiosis. NO sup-inf3 (5 mM) lowered while naringenin raised the nodulation status (nodule numbers and weight) and nodule efficiency (C2H2 reduction activity). However, the inhibitory effect of NO sup-inf3 was to some extent alleviated when applied in combination with naringenin. The plant biomass was increased by the application of NO sup-inf3 and naringenin, either alone or in combination, while a higher root: shoot ratio was observed only in the naringenin-treated plants. Root flavonoids are known to regulate the expression of nod genes; their high-performance liquid chromatography profile was influenced in different ways by NO sup-inf3 and naringenin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Cho MJ, Harper JE (1991a) Effect of inoculation and nitrogen on isoflavonoid concentration in wild type and nodulation mutant soybean roots. Plant Physiol 95:435–442

    Google Scholar 

  • Cho MJ, Harper JE (1991b) Effect of localized nitrate application on isoflavonoid concentration and nodulation in split root systems of wild type and nodulation mutant soybean plants. Plant Physiol 95:1106–1112

    Google Scholar 

  • Dahiya JS, Khurana AL (1981) ‘Chillum jar’ — a better technique for screening of rhizobia under summer conditions. Plant and Soil 83:299–302

    Google Scholar 

  • Dusha I, Bakos A, Kondorosi A, De Bruijn FJ, Schell J (1989) The Rhizobium meliloti early nodulation genes (nod ABC) are nitrogen regulated: Isolation of a mutant strain with efficient nodulation capacity of alfalfa in presence of ammonium. Mol Gen Genet 219:89–96

    Google Scholar 

  • Firmin JL, Wilson KE, Rossen L, Johnston AWB (1986) Flavonoid activation of nodulation genes in Rhizobium reversed by other compounds present in plants. Nature (London) 324:90–92

    Google Scholar 

  • Fisher RF, Long SR (1992) Rhizobium-plant signal exchange. Nature (London) 357:655–660

    Google Scholar 

  • Fred CB, Baldwin FN, MoCoy E (1932) Root nodule bacteria and leguminous plants. In: Studies in sciences, University of Wisconsin Press, London, p 343

    Google Scholar 

  • Gibson AH (1974) Comparison of growing legume as a symbiotic association. Proc Indian Natl Sci Acad 40 B:741–767

    Google Scholar 

  • Hardy RWF, Hosten RD, Jackson EK, Burns RC (1968) The acetylene-ethylene assay for nitrogen fixation; laboratory and field evaluation. Plant Physiol 43:1185–1207

    Google Scholar 

  • Hartwig UA, Joseph CM, Phillips DA (1991) Flavonoids released naturally from alfalfa seeds enhance growth rate of Rhizobium meliloti. Plant Physiol 95:797–803

    Google Scholar 

  • Jain V, Garg N, Nainawatee HS (1990) Naringenin enhanced efficiency of Rhizobium meliloti — alfalfa symbiosis. World J Appl Microbiol Biotechnol 6:434–436

    Google Scholar 

  • Jain V, Garg N, Nainawatee HS (1991) Influence of rhizopheric application of syringaldehyde on rhizobia-mungbean (Vigna radiata) symbiosis. Biochem Physiol Pflanz 187:331–336

    Google Scholar 

  • Jain V, Garg N, Nainawatee HS (1992) Influence of flavonoids on nodulation and carbon partitioning in pea-Rhizobium symbiosis. J Plant Biochem Biotechnol 1:23–26

    Google Scholar 

  • Jain V, Garg N, Nainawatee HS (1993) Effect of nitrate on Bradyrhizobium mungbean (Vigna radiata) symbiosis in presence of syringaldehyde. J Plant Biochem Biotechnol 2:25–27

    Google Scholar 

  • Kapulnik Y, Joseph CM, Phillips DA (1987) Flavone limitations to root nodulation and symbiotic nitrogen fixation in alfalfa. Plant Physiol 84:1193–1196

    Google Scholar 

  • Raghuwanshi A, Dudeja SS, Khurana AL (1994) Effect of temperature on flavonoid production in pigeonpea [Cajanus cajan (L) Mill sp] in relation to nodulation. Biol Fertil Soils 17:314–316

    Google Scholar 

  • Redmond JW, Bailey MN, Djordjevic MA, Innes RW, Kuempel PL, Rolfe BG (1986) Flavones induce expression of nodulation genes in Rhizobium. Nature (London) 232:623–625

    Google Scholar 

  • Sawhney V, Amarjit, Singh R (1985) Effect of applied nitrate on growth and nitrogen fixation in Cicer arietinum. Plant and Soil 86:233–240

    Google Scholar 

  • Sherwood JE, Truchet GL, Dazzo FB (1984) Effect of nitrate supply on the in vivo synthesis and distribution of trifoliin A, a Rhizobium trifolii binding lectin in Trifolium repens seedlings. Planta 162:540–547

    Google Scholar 

  • Sloger C (1969) Symbiotic effectiveness and nitrogen fixation in nodulated soybean. Plant Physiol 44:1166–1168

    Google Scholar 

  • Smith H (1972) The photo control of flavonoid biosynthesis. In: Mitrakos K, Shropshire W Jr (eds) Phytochromes. Academic Press, New York, pp 433–481

    Google Scholar 

  • Streeter JG (1985) Nitrate inhibition of legume nodule growth and activity. 1. Long term studies with a continuous supply of nitrate. Plant Physiol 77:321–324

    Google Scholar 

  • Streeter JG (1988) Inhibition of legume nodule formation and nitrogen fixation by nitrate. Crit Rev Plant Sci 7:1–23

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bandyopadhyay, A.K., Jain, V. & Nainawatee, H.S. Nitrate alters the flavonoid profile and nodulation in pea (Pisum sativum L.). Biol Fertil Soils 21, 189–192 (1996). https://doi.org/10.1007/BF00335933

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00335933

Key words

Navigation