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A temporally regulated promoter from Bacillus subtilis is transcribed only by an RNA polymerase with a 37,000 dalton sigma factor

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Summary

A 1,250 base pair Bacillus subtilis chromosomal HindIII restriction fragment (S fragment) has been cloned into the B. subtilis expression-probe plasmid pGR71. The S fragment induces the expression of the pGR71 chloramphenicol resistance gene shortly after the initiation of sporulation. The transcriptional promoter responsible for the expression of this temporally regulated genetic element has been identified and mapped in vitro. This promoter is recognized exclusively by the minor B. subtilis RNA polymerase that contains the 37,000 dalton sigma factor.

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, University of California, Davis, 95616, Davis, California, USA

    David S. Goldfarb, Sui-Lam Wong, Toshiaki Kudo & Roy H. Doi

Authors
  1. David S. Goldfarb
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  2. Sui-Lam Wong
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  3. Toshiaki Kudo
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  4. Roy H. Doi
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Communicated by G. O'Donovan

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Goldfarb, D.S., Wong, SL., Kudo, T. et al. A temporally regulated promoter from Bacillus subtilis is transcribed only by an RNA polymerase with a 37,000 dalton sigma factor. Mol Gen Genet 191, 319–325 (1983). https://doi.org/10.1007/BF00334833

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  • DOI: https://doi.org/10.1007/BF00334833

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