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Hybridization selection and translation of T-DNA encoded mRNAs from octopine tumors

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Summary

To characterize the functions of T-DNA derived transcripts in plant tumor cells, we isolated these RNAs by a highly sensitive hybridization selection procedure and investigated whether they are translatable into proteins in wheat germ extracts. Results with two independent tobacco cell lines transformed with octopine plasmids pTiA6 and pTiB6S3 show that the cells contained at least three translatable T-DNA derived mRNAs. Each represented 0.0001% or less of the total mRNA activity in polyribosomal RNA. All were detected in polyadenylated as well as in nonpolyadenylated RNA fractions, and translation was inhibited by the cap analogue pm7G. Two of the proteins were encoded close to the right and the left end of the T-DNA (proteins Mr 39,000 and 14,000, respectively); the third protein was derived from the middle of the T-DNA. The results indicate that genetic manipulation of plants by Agrobacteria involves transfer of several genes which are expressed into proteins in eucaryotic cells.

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Authors and Affiliations

  1. Abt. Schell, Max-Planck-Institut für Züchtungsforschung, D-5000, Köln 30, Germany

    Gudrun Schröder & Joachim Schröder

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  1. Gudrun Schröder
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  2. Joachim Schröder
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Communicated by G. Melchers

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Schröder, G., Schröder, J. Hybridization selection and translation of T-DNA encoded mRNAs from octopine tumors. Molec Gen Genet 185, 51–55 (1982). https://doi.org/10.1007/BF00333789

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  • DOI: https://doi.org/10.1007/BF00333789

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