Summary
Versatile cloning vectors were constructed employing a runaway replication mutant of the bacteriocinogenic plasmid CloDF13. These vectors can, under conditions where protein synthesis is not inhibited, be amplified in Escherichia coli to high levels by elevating the temperature and are therefore useful for the production of large quantities of DNA and protein. Since the constructed shuttle vectors, which harbour at least six unique restriction endonucleases sites, replicate in E. coli, Enterobacter cloacae, Staphylococcus aureus and a variety of Bacilli, they are applicable for the genetic engineering of both gramnegative and gram-positive bacteria.
Similar content being viewed by others
References
Anagnostopoulos C, Spizizen J (1961) Requirements for transformation in Bacillus subtilis. J Bacteriol 81:741–746
Andreoli PM, Brandsma RA, Veltkamp E, Nijkamp HJJ (1978) Isolation and characterization of a CloDF13::Tn901 plasmid mutant with thermosensitive control of DNA replication. J Bacteriol 135:612–621
Aubert E, Fargett F, Fouet A, Klier A, Rapoport G (1982) Use of a bifunctional cosmid for cloning large DNA fragments of B. subtilis. In: Ganesan AT, Chang S, Hoch JA (eds) Molecular cloning and gene regulation in Bacilli. Academic Press, New York, pp 11–24
Bittner M, Vapnek D (1981) Versatile cloning vectors derived from the runaway-replication plasmid pKN402. Gene 15:319–329
Bolivar F, Rodriguez RL, Greene PJ, Betlach MC, Heyneker HL, Boyer HW, Crosa JH, Falkow S (1977) Construction and characterization of new cloning vehicles, II. A multipurpose cloning system. Gene 2:95–113
Cami B, Kourilsky P (1978) Screening of cloned recombinant DNA in bacteria by in situ colony hybridization. Nucleic Acids Res 5:2381–2390
Canosi U, Morelli G, Trautner TA (1978) The relationship between molecular structure and transformation efficiency of some S. aureus plasmids isolated from B. subtilis. Mol Gen Genet 166:259–267
Curtiss III R, Caro LG, Allison DP, Stallions DR (1969) Early stages of conjugation in Escherichia coli. J Bacteriol 100:1091–1104
Dagert M, Ehrlich SD (1979) Prolonged incubation in calcium chloride improves the competence of E. coli cells. Gene 6:23–28
Ehrlich SD, Niaudet B, Michel B (1981) Use of plasmids from Staphylococcus aureus for cloning of DNA in Bacillus subtilis. Curr Top Microbiol Immunol 96:19–29
Gryczan TJ, Contente S, Dubnau D (1978) Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J Bacteriol 134:318–329
Hakkaart MJJ, Veltkamp E, Nijkamp HJJ (1981a) Protein H encoded by plasmid CloDF13 involved in lysis of the bacterial host. I. Localization of the gene and identification and subcellular localization of the gene H product. Mol Gen Genet 183:318–325
Hakkaart MJJ, Veltkamp E, Nijkamp HJJ (1981b) Protein H encoded by plasmid CloDF13 involved in lysis of the bacterial cell. II. Functions and regulation of synthesis of the gene H product. Mol Gen Genet 183:326–332
Hakkaart MJJ, Veltkamp E, Nijkamp HJJ (1082) Maintenance of the bacteriocinogenic plasmid CloDF13 in E. coli cells. II. Specific recombination functions involved in plasmid maintenance. Mol Gen Genet 188:338–344
Hohn B, Collins J (1980) A small cosmid for efficient cloning of large DNA fragments. Gene 11:291–298
Hollenberg CP, Das C, van den Berg JA, de Leeuw A (1983) Cloning system for Kluyveromyces species. European Patent Office, European Patent Application no 8202091
Holmes DS, Quigley M (1981) A rapid boiling method for the preparation of bacterial plasmids. Anal Biochem 114:193–197
Ish-Horowicz D, Burke JF (1981) Rapid and efficient cosmid cloning. Nucleic Acids Res 9:2989–2998
Kornberg A (1974) DNA synthesis. Freeman, San Francisco, p 17
Kos A, Broekhuijsen MP, van Gorcom RFM, van Rotterdam J, Enger-Valk BE, van Putten AJ, Veltkamp E, van den Hondel CAMJJ, Pouwels PH (1984) Construction of vectors suitable for efficient expression of genes in E. coli. In: Houwink EH, van der Meer RR (eds) Progress in Industrial Microbiology 20. Elsevier Science Publishers, Amsterdam, pp 415–424
Loenen WAM, Brammer WJ (1980) A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes. Gene 10:249–259
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory Manual. Cold Spring Harbor Laboratory Cold Spring Harbor, NY
Miller JH (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Moormann RJM, den Dunnen JT, Mulleners LJSM, Andreoli PM, Bloemendal H, Schoenmakers JGG (1983) Strict co-linearity of genetic and protein folding domains in an intragenically duplicated rat lens γ-crystallin gene. J Mol Biol 171:353–368
Oudega B, Stegehuis F, van Tiel-Menkveld GJ, de Graaf FK (1982) Protein H encoded by plasmid CloDF13 is involved in excretion of cloacin DF13. J Bacteriol 150:1115–1121
Priest FG (1977) Extracellular enzyme synthesis in the genus Bacillus. Bacteriol Rev 41:711–753
Remaut E, Tsao H, Fiers W (1983) Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replication. Gene 22:103–113
Scheer-Abramowitz J, Gryczan TJ, Dubnau D (1981) Origin and mode of replication of plasmids pE194 and pUB110. Plasmid 6:67–77
Snijders A, van Putten AJ, Veltkamp E, Nijkamp HJJ (1983) Localization and nucleotide sequence of the bom region of CloDF13. Mol Gen Genet 192:444–451
Sninsky JJ, Uhlin BE, Gustafsson P, Cohen SN (1981) Construction and characterization of a novel two plasmid system for accomplishing temperature regulated, amplified expression of cloned adventitious genes in E. coli. Gene 16:116–122
Stuitje AR, Veltkamp E, van den Elzen PJM, Nijkamp HJJ (1978) In vitro construction of deletion mutants of the bacteriocinogenic plasmid CloDF13. Nucleic Acids Res 5:1801–1820
Stuitje AR, Spelt CE, Veltkamp E, Nijkamp HJJ (1981) Identification of mutations affecting replication control of plasmid CloDF13. Nature 290:264–267
Thorne CG, Stull HB (1966) Factors affecting transformation of Bacillus licheniformis. J Bacteriol 91:1012–1020
Twigg AJ, Sherratt D (1980) Trans-complementable copy-number mutants of plasmid ColE1. Nature 283:216–218
Uhlin BE, Molin S, Gustafsson P, Nordstrom K (1979) Plasmids with temperature-dependent copy number for amplification of cloned genes and their products. Gene 6:91–106
Uhlin BE, Schweickart V, Clark AJ (1983) New runaway-replication plasmid cloning vectors and suppression of runaway replication by novobiocin. Gene 22:255–265
Veltkamp E, Stuitje AR (1981) Replication and structure of the bacteriocinogenic plasmids CloDF13 and ColE1. Plasmid 5:76–99
Young FE (1980) Impact of cloning in Bacillus subtilis on fundamental and industrial microbiology. J Gen Microbiol 119:1–15
Yuki S (1967) Genetic studies on amylase of different electrophoretic mobility produced by strains of Bacillus subtilis. Jpn J Genet 42:251–261
Author information
Authors and Affiliations
Additional information
Communicated by H. Böhme
Rights and permissions
About this article
Cite this article
Andreoli, P.M. Versatile Escherichia coli-Bacillus shuttle vectors derived from runaway replication plasmids related to CloDF13. Mol Gen Genet 199, 372–380 (1985). https://doi.org/10.1007/BF00330745
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00330745