Summary
Transfer of chromosomal genes between Escherichia coli K12 and Enterobacter aerogenes was carried out by P1-mediated transduction as well as by transformation of genes cloned in vitro on plasmid vectors. The efficient expression of E. coli genes in E. aerogenes probably reflects the existance of a poor restriction system in the latter, and suggests that this strain might be useful as a recipient of genetic information from E. coli.
References
Cabello F, Timmis K, Cohen SN (1976) Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons. Nature (London) New Biol 259:285–289
Clewell DB (1972) Nature of ColE1 plasmid replication in Escherichia coli in the presence of chloramphenicol. J Bacteriol 110:667–678
Davis BD (1950) Studies on nutritionally deficient bacterial mutants, isolated by means of penicillin. Experientia 6:41–50
Goldberg RB, Bender RA, Streicher SL (1974) Direct selection for P1-sensitive mutants of enteric bacteria. J Bacteriol 118:810–814
Lederberg EM, Cohen SN (1974) Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. J Bacteriol 119:1072–1074
MacDonnel MW, Simon MN, Studer FW (1977) Analysis of restriction fragments of T7 DNA determination of molecular weight by electrophoresis in neutral and alkaline gels. J Mol Biol 110:119–146
Michaeli S, Ron EZ, Cohen G (1981) Construction and physical mapping of plasmids containing the met A gene of Escherichia coli K-12. Mol Gen Genet 182:349–354
Miller JH (1972) In: Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor NY
Schleif R (1980) Assaying of organisms for the presence of restriction endonuclease. In: Methods in enzymology, vol 65, part I. Academic Press, New York, pp 19–23
Tyler BM, Goldberg RB (1976) Transduction of chromosomal genes between enteric bacteria by bacteriophage P1. J Bacteriol 125:1105–1111
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Communicated by K. Isono
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Michaeli, S., Ron, E.Z. Gene transfer between Escherichia coli and Enterobacter aerogenes . Mol Gen Genet 190, 179–181 (1983). https://doi.org/10.1007/BF00330344
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DOI: https://doi.org/10.1007/BF00330344