Summary
The number of antibody molecules on individual erythrocytes was counted in A1, A2, A3 B and A group individuals using immunoautoradiography (IAR) and monoclonal IgM anti-A1. Quantitation was also done for A group pregnant women. The number of antibody molecules on different red cells of an individual varied widely. Gross variations were also noted in cells of different individuals from one and the same group. The mean values of the uptake of the number of antibody molecules showed the following range A1>A2>Ax>A3B. When compared to the average for total A1 adults, red cells of pregnant women and newborn infants showed a 10.7% and 19.7% reduction respectively, in antibody uptake. The mean number of antibody molecules per A1 adult red cells was 5.6±3×104, while A2 had 0.85±0.35×104 molecules, thus showing a significant quantitative variation.
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References
Bhatia HM, Boyd WC, Brown R (1967) Serological studies of Snail (Otela lactas) anti-A: simple purification method. Transfusion 7: 53–59
Bhatia HM, Kim YC, Boyd WC (1968) Serological and immunochemical characterisation of the Lima bean anti-A lectin. Vox sang 15: 278–286.
Boyd WC, Bhatia HM, Diamond MA, Matsubara S (1962) Quantitative study of the combination of Lima bean lectin with human erythrocytes. J Immunol 89: 463–470
Carton JP, Garbal A, Hughes-Jones NC, Slamon C (1974) “Weak A” phenotype. Relationship between red cell agglutinability and antigen site density Immunology 27: 723–727.
Cohan F, Zuelzer WW (1965) Interrelationship of the various subgroups of the blood A: study with immunofluorescence. Transfusion 5: 223–229
Dalal RM, Sathe MS, Bhatia HM (1982): ABH blood group antigens during pregnancy and in women on hormonal contraceptives. Indian J Med Res 76: 201–206
Economidou J, Hughes-Jones NC, Gardner B (1967) Quantitative measurements concerning A and B antigen sites. Vox Sang 12: 321–328
Economidou J, Hughes-Jones NC, Gardner B (1967) The functional activities of IgG and IgM anti-A. Immunology 13: 227–234
Greenbury CL, Moore DH, Nun LAC (1963) Reaction of 7 S and 19 S components of immune rabbit antisera with human group A and AB red cells. Immunology 6: 421–433
Hamilton RG, Solman A, Button M (1980) Protein radioiodination in a radioassay laboratory: evaluation of commercial Na 125I reagents and related biohazards. J Immunoassay 1 (4): 435–448
Lopez M, Banali J, Cartron JP, Salmon C (1980) some notes on specificity of anti A1 reagents. Vox Sang 39: 271–276
Majdic O. Knapp W, Vetterkain M, Mayy WR, Speissar P (1979) Hybridomas secreting monoclonal antibodies to human group A erythrocytes. Immunobiology 156: 226–227
Matsukura Y (1972) Demonstration of ferritin labelled antibodies bound to human erythrocytes fixed with glutaraldehyde Vox Sang 22: 549–553
McConahey PJ, Dixon FJ (1966): A method of trace iodination of proteins for immunological studies. Int Arch Allergy Appl Immunol 29: 185–189
Mittal KK, Micky MR, Singal DP, Terasaki PI (1968) Serotyping for homotransplantation. XVIII Refinement of microdroplet lymphocyte cytotoxicity test. Transplantation 6: 913–927
Mollison PL (1979) Blood transfusion in clinical medicine, 6th ed. ELBS and Blackwell Scientific Publications, Oxford, PP 241, 428
Ringelmann RW, Opferkuch W, Röllinghoff M, Loos M (1969) Komplement Messungen mit Hilfe des Microlitersystems. Z Med Mikrobiol Immunol 154: 329–343
Thiel E, Dörmer P, Rodt H, Thierfelder S (1975) Quantitative immunoautoradiography at the cellular level. I. Design of a microphotometric method to quantitate membrane antigens on single cells using 125I labelled antibodies J Immunol Methods 6: 317–330
Thiel E, Dörmer P, Ruppelt W, Thierfelder S (1976) Quantitative immunoautoradiography at the cellular level. II. Absolute measurements using labelled standard cells, as a source of reference. J Immunol Methods 12: 237–251
Van Gastel C, Dewit CD (1970) The effect of red cell age on its agglutinability by anti-A. Vox Sang 19: 105–112
Voak D, Lodge TW (1968) The role of H in the development of A. Vox Sang 15: 345–352
Voak D. Williams MA (1971) An explanation of the failure of the direct antiglobulin test to detect erythrocyte sensitization in AB0 Haemolytic disease of the newborn and observations on pinocytosis of IgG anti-A antibodies by infant (cord) red cells. Br J Haematol 20: 9–23
Voak D, Sacks S, Alderson T, Takei F, Lemmox E, Jervis J, Milstein C, Darborough J (1980) Monoclonal anti-A from a hybrid myeloma: evaluation as a blood grouping reagent. Vox Sang 39: 134–140
Voak D, Lennox E, Sachs S, Milstein C, Darnborough J (1982) Monoclonal anti-A and anti-B: development as cost effective reagents. Med Lab Sci 39: 109–122
Williams MA, Voak D, (1972) Studies on ferritin labelled Dolichos biflorus lectin on the numbers and distribution of a sites on A1 and A2 erythrocytes and its specificity and enhancement by enzymes. Br J Haematol 23: 427–441
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Gupte, S.C., Thiel, E., Majdic, O. et al. Quantitation of antibody uptake on a group erythrocytes using immunoautoradiography and monoclonal IgM anti-A. Blut 51, 251–258 (1985). https://doi.org/10.1007/BF00320519
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DOI: https://doi.org/10.1007/BF00320519