Skip to main content
Log in

A study on skeletal myogenic cell movement in the developing avian limb bud

  • Published:
Anatomy and Embryology Aims and scope Submit manuscript

Summary

Quail limb mesenchyme containing myogenic cells of somitic origin were transplanted into chick limb buds to determine whether cell movement might play a role in avian limb myogenesis. In general, cell displacement was not detected 1-day after implantation: all quail cells were found at the graft site. Migration was evident 2-days after implantation but not all cell types were capable of movement; myogenic cells were very invasive while chondrocytes were relatively immobile. The spreading of myogenic cells was discernible up to 4-days after implantation and specifically in a proximodistal direction towards the apex of the limb.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Cairns JM (1966) Development of grafts from mouse embryos to the wing bud of the chick embryo. Dev Biol 12:36–52

    Google Scholar 

  • Chevallier A, Kieny M (1982) On the role of the connective tissue in the patterning of the chick limb musculature. Wilhelm Roux's Archives 191:277–280

    Google Scholar 

  • Chevallier A, Kieny M, Mauger A (1976) Sur l'origine de la musculature de l'aile chez les oiseaux. C R Acad Sci Paris Serie D 282:309–311

    Google Scholar 

  • Chevallier A, Kiney M, Mauger (1977) Limb-somite relationship: effect of removal of somitic mesoderm on the wing musculature. J Embryol Exp Morphol 43:263–278

    Google Scholar 

  • Christ B, Jacob HJ, Jacob M (1974) Über den Ursprung der Flügelmuskulatur. Experimentelle Untersuchungen mit Wachter und Hühnerembryonen. Experentia 30:1446–1449

    Google Scholar 

  • Christ B, Jacob HJ, Jacob M (1977) Experimental analysis of the origin of the wing musculature in avian embryos. Anat Embryol 150:171–186

    Google Scholar 

  • Christ B, Jacob HJ, Jacob M (1983) On the origin and determination of chick limb mesenchymal cells. In: Teratology of the limb. Walter de Gruyter, NY, pp 67–77

    Google Scholar 

  • Ede DA, Gumpel-Pinot M, Flint OP (1984) Orientated movement of myogenic cells in the avian limb bud and its dependence on presence of the apical ectodermal ridge. In: Kemp RB, Hinchliffe JR (eds) Matrices and cell differentiation. Alan R Liss, NY, pp 427–488

    Google Scholar 

  • Fisher M, Solursh M (1979) The influence of local environment on the organization of mesenchymal cells. J Embryol Exp Morphol 49:295–306

    Google Scholar 

  • Gumpel-Pinot M, Ede DA, Flint OP (1984) Myogenic cell movement in the developing avian limb bud in presence and absence of the apical ectodermal ridge (AER). J Embroyl Exp Morphol 80:105–125

    Google Scholar 

  • Hamburger C, Hamilton HL (1951) A series of normal stages in the development of the chick embryo. J Morphol 88:49–82

    Google Scholar 

  • Humason GL (1972) Animal tissue technique. WH Freeman

  • Jacob HJ, Christ B, Grim M (1983) Problems of muscle pattern formation and of neuromuscular relations in avian limb development. In: Limb Development and Regeneration, Part B. Alan R Liss, NY, pp 33–41

    Google Scholar 

  • Kieny M, Pautou MP, Chevallier A, Mauger A (1986) Spatial organization of the developing limb musculature in birds and mammals. Bibl Anat 29:65–90

    Google Scholar 

  • Kujawa MJ, Tepperman K (1983) Culturing chick muscle cells on glycosanoglycan substrates: attachment and differentiation. Dev Biol 99:277–286

    Google Scholar 

  • Le Douarin N (1972) A biological cell labelling technique and its use in experimental embryology. Dev Biol 30:217–233

    Google Scholar 

  • Lee KKH (1986) A study on the capacity of normal and talpid 3 mutant myogenic cells to migrate in fowl embryonic wing buds. PhD Thesis, University of Glasgow

  • Lee KKH, Ede DA (1989) The capacity of normal and talpid 3 mutant fowl myogenic cells to migrate in quail limb buds. Anat Embryol 179:395–402

    Google Scholar 

  • Moscona A, Moscona H (1952) The dissociation and aggregation of cells from organ rudiments of the early chick embryl. J Anat 86:287–301

    Google Scholar 

  • Oster GF, Murray JD, Harris AK (1983) Mechanical aspects of mesenchymal morphogenesis. J Embryol Exp Morphol 78:83–125

    Google Scholar 

  • Searls RL (1967) The role of the cell migration in the development of the embryonic chick limb bud. J Exp Zool 166:39–50

    Google Scholar 

  • Searls RL, Smith AA (1982) Evidence that ectoderm influences the differentiation of muscle in the limb of the embryonic chick. J Exp Zool 202:343–351

    Google Scholar 

  • Seed J, Hauschka SD (1984) Temporal separation of the migration of distinct myogenic precursor populations into the developing chick wing bud. Dev Biol 106:389–393

    Google Scholar 

  • Singley CT, Solursh M (1981) The spatial distribution of hyaluronic acid and mesenchymal condensation in the embryonic chick wing. Dev Biol 84:102–120

    Google Scholar 

  • Solursh M (1984) The migratory capacity of myogenic cells in vitro. Dev Biol 102:509–513

    Google Scholar 

  • Solursh M, Meiers S (1986) The distribution of somite-derived myogenic cells during early development of the wing bud. In: Bellairs R, Ede DA, Lash JW (eds) Somites in developing embryos. NATO ASI Series A, vol 118. Plenum Press, New York, pp 277–287

    Google Scholar 

  • Steinberg MS (1970) Does differential adhesion govern self-assembly process in histogenesis? Equilibrium configurations and the emergence of a hierarchy among populations of embryonic cells. J Exp Zool 73:295–343

    Google Scholar 

  • Tickle C, Goodman M, Wolpert L (1978) Cell contacts and sorting out in vitro: the behaviour of some embryonic tissues implanted into the developing chick wing. J Embryol Exp Morphol 48:225–237

    Google Scholar 

  • Turner DC, Lawton J, Dollenmeier P, Ehrismann R, Chiquet M (1983) Guidance of myogenic cell migration by oriented deposits of fibronectin. Dev Biol 95:497–504

    Google Scholar 

  • Venkatasubramanian K, Solursh M (1984) Chemotactic behavior of myoblasts. Dev Biol 104:428–433

    Google Scholar 

  • Wachtler F (1984) On the migration of epidermal melanoblasts in the avian wing bud. Anat Embryol 170:307–312

    Google Scholar 

  • Wachtler F, Christ B, Jacob HJ (1981) On the determination of mesodermal tissues in the avian embryonic wing bud. Anat Embryol 161:283–289

    Google Scholar 

  • Wachtler F, Christ B, Jacob HJ (1982) Grafting experiments in determination and migratory behaviour of presomitic, somitic and somatopleural cells in avian embryos. Anat Embryol 164:369–378

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, K.K.H., Ede, D.A. A study on skeletal myogenic cell movement in the developing avian limb bud. Anat Embryol 180, 293–300 (1989). https://doi.org/10.1007/BF00315887

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00315887

Key words

Navigation