Summary
Quail limb mesenchyme containing myogenic cells of somitic origin were transplanted into chick limb buds to determine whether cell movement might play a role in avian limb myogenesis. In general, cell displacement was not detected 1-day after implantation: all quail cells were found at the graft site. Migration was evident 2-days after implantation but not all cell types were capable of movement; myogenic cells were very invasive while chondrocytes were relatively immobile. The spreading of myogenic cells was discernible up to 4-days after implantation and specifically in a proximodistal direction towards the apex of the limb.
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References
Cairns JM (1966) Development of grafts from mouse embryos to the wing bud of the chick embryo. Dev Biol 12:36–52
Chevallier A, Kieny M (1982) On the role of the connective tissue in the patterning of the chick limb musculature. Wilhelm Roux's Archives 191:277–280
Chevallier A, Kieny M, Mauger A (1976) Sur l'origine de la musculature de l'aile chez les oiseaux. C R Acad Sci Paris Serie D 282:309–311
Chevallier A, Kiney M, Mauger (1977) Limb-somite relationship: effect of removal of somitic mesoderm on the wing musculature. J Embryol Exp Morphol 43:263–278
Christ B, Jacob HJ, Jacob M (1974) Über den Ursprung der Flügelmuskulatur. Experimentelle Untersuchungen mit Wachter und Hühnerembryonen. Experentia 30:1446–1449
Christ B, Jacob HJ, Jacob M (1977) Experimental analysis of the origin of the wing musculature in avian embryos. Anat Embryol 150:171–186
Christ B, Jacob HJ, Jacob M (1983) On the origin and determination of chick limb mesenchymal cells. In: Teratology of the limb. Walter de Gruyter, NY, pp 67–77
Ede DA, Gumpel-Pinot M, Flint OP (1984) Orientated movement of myogenic cells in the avian limb bud and its dependence on presence of the apical ectodermal ridge. In: Kemp RB, Hinchliffe JR (eds) Matrices and cell differentiation. Alan R Liss, NY, pp 427–488
Fisher M, Solursh M (1979) The influence of local environment on the organization of mesenchymal cells. J Embryol Exp Morphol 49:295–306
Gumpel-Pinot M, Ede DA, Flint OP (1984) Myogenic cell movement in the developing avian limb bud in presence and absence of the apical ectodermal ridge (AER). J Embroyl Exp Morphol 80:105–125
Hamburger C, Hamilton HL (1951) A series of normal stages in the development of the chick embryo. J Morphol 88:49–82
Humason GL (1972) Animal tissue technique. WH Freeman
Jacob HJ, Christ B, Grim M (1983) Problems of muscle pattern formation and of neuromuscular relations in avian limb development. In: Limb Development and Regeneration, Part B. Alan R Liss, NY, pp 33–41
Kieny M, Pautou MP, Chevallier A, Mauger A (1986) Spatial organization of the developing limb musculature in birds and mammals. Bibl Anat 29:65–90
Kujawa MJ, Tepperman K (1983) Culturing chick muscle cells on glycosanoglycan substrates: attachment and differentiation. Dev Biol 99:277–286
Le Douarin N (1972) A biological cell labelling technique and its use in experimental embryology. Dev Biol 30:217–233
Lee KKH (1986) A study on the capacity of normal and talpid 3 mutant myogenic cells to migrate in fowl embryonic wing buds. PhD Thesis, University of Glasgow
Lee KKH, Ede DA (1989) The capacity of normal and talpid 3 mutant fowl myogenic cells to migrate in quail limb buds. Anat Embryol 179:395–402
Moscona A, Moscona H (1952) The dissociation and aggregation of cells from organ rudiments of the early chick embryl. J Anat 86:287–301
Oster GF, Murray JD, Harris AK (1983) Mechanical aspects of mesenchymal morphogenesis. J Embryol Exp Morphol 78:83–125
Searls RL (1967) The role of the cell migration in the development of the embryonic chick limb bud. J Exp Zool 166:39–50
Searls RL, Smith AA (1982) Evidence that ectoderm influences the differentiation of muscle in the limb of the embryonic chick. J Exp Zool 202:343–351
Seed J, Hauschka SD (1984) Temporal separation of the migration of distinct myogenic precursor populations into the developing chick wing bud. Dev Biol 106:389–393
Singley CT, Solursh M (1981) The spatial distribution of hyaluronic acid and mesenchymal condensation in the embryonic chick wing. Dev Biol 84:102–120
Solursh M (1984) The migratory capacity of myogenic cells in vitro. Dev Biol 102:509–513
Solursh M, Meiers S (1986) The distribution of somite-derived myogenic cells during early development of the wing bud. In: Bellairs R, Ede DA, Lash JW (eds) Somites in developing embryos. NATO ASI Series A, vol 118. Plenum Press, New York, pp 277–287
Steinberg MS (1970) Does differential adhesion govern self-assembly process in histogenesis? Equilibrium configurations and the emergence of a hierarchy among populations of embryonic cells. J Exp Zool 73:295–343
Tickle C, Goodman M, Wolpert L (1978) Cell contacts and sorting out in vitro: the behaviour of some embryonic tissues implanted into the developing chick wing. J Embryol Exp Morphol 48:225–237
Turner DC, Lawton J, Dollenmeier P, Ehrismann R, Chiquet M (1983) Guidance of myogenic cell migration by oriented deposits of fibronectin. Dev Biol 95:497–504
Venkatasubramanian K, Solursh M (1984) Chemotactic behavior of myoblasts. Dev Biol 104:428–433
Wachtler F (1984) On the migration of epidermal melanoblasts in the avian wing bud. Anat Embryol 170:307–312
Wachtler F, Christ B, Jacob HJ (1981) On the determination of mesodermal tissues in the avian embryonic wing bud. Anat Embryol 161:283–289
Wachtler F, Christ B, Jacob HJ (1982) Grafting experiments in determination and migratory behaviour of presomitic, somitic and somatopleural cells in avian embryos. Anat Embryol 164:369–378
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Lee, K.K.H., Ede, D.A. A study on skeletal myogenic cell movement in the developing avian limb bud. Anat Embryol 180, 293–300 (1989). https://doi.org/10.1007/BF00315887
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DOI: https://doi.org/10.1007/BF00315887