Summary
Eight sterile mutants, which regain their fertility upon reactivation of an inactivated UGA suppressor allele of the serine tRNA gene sup3, are shown to carry UGA nonsense alleles of two established ste genes, ste1 (one mutant) and ste6 (two mutants), and of two novel genes, ste9 (four mutants) and ste10 (one leaky mutant of ras1 -/ste5-like cell morphology). The mutant alleles of ste1 and ste9 lead to a defect in both conjugation and meiosis, whereas those of ste6 and ste10 affect mating only. Two of the four genes map to chromosome I, ste1 in the left arm 6 cM distal of ura1, and ste9 in the right arm 3 cM distal of ade2. The ste10 and ste6 genes are located in the right arms of chromosomes II and III, respectively, the former 4 cM distal of trp1 and the latter 1 cM proximal or distal of trp3.
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Amstutz H, Munz P, Heyer W-D, Leupold U, Kohli J (1985) Cell 40:879–886
Bähler J, Schuchert P, Grimm C, Kohli J (1991) Curr Genet (in press)
Beach D (1983) Nature 305:682–688
Beach D, Klar AJS (1984) EMBO J 3:603–610
Beach D, Rodgers L, Gould J (1985) Curr Genet 10:297–311
Bresch C, Müller G, Egel R (1968) Mol Gen Genet 102:301–306
Costello G, Rodgers L, Beach D (1986) Curr Genet 11:119–125
Egel R (1973) Mol Gen Genet 122:339–343
Egel R (1989) In: Nasim A, Young P, Johnson BF (eds) Molecular biology of the fission yeast. Academic Press, San Diego, pp 71–73
Egel R, Egel-Mitani M (1974) Exp Cell Res 88:127–134
Egel R, Willer M, Nielsen O (1989) Curr Genet 15:407–410
Fukui Y, Yamamoto M (1988) Mol Gen Genet 215:26–31
Fukui Y, Kozasa T, Kaziro Y, Takeda T, Yamamoto M (1986a) Cell 44:329–336
Fukui Y, Kaziro Y, Yamamoto M (1986b) EMBO J 5:1991–1993
Fukui Y, Miyake S, Satoh M, Yamamoto M (1989) Mol Cell Biol 9:5617–5622
Girgsdies O (1982) Curr Genet 6:223–227
Gutz H, Heslot H, Leupold U, Loprieno N (1974) In: King RC (ed) Handbook of Genetics, vol 1. Plenum Press, New York, pp 395–446
Hofer F, Hollenstein H, Janner F, Minet M, Thuriaux P, Leupold U (1979) Curr Genet 1:45–61
Iino Y, Yamamoto M (1985a) Mol Gen Genet 198:416–421
Iino Y, Yamamoto M (1985b) Proc Natl Acad Sci US 82:2447–2451
Kitamura K, Nakagawa T, Shimoda C (1990) Curr Genet 18: 315–321
Kohli J, Munz P, Aebi R, Amstutz H, Gysler C, Heyer W-D, Lehmann L, Schuchert P, Szankasi P, Thuriaux P, Leupold U, Bell J, Gamulin V, Hottinger H, Pearson D, Söll D (1984) Cold Spring Harbor Symp Quant Biol 49:31–40
Kohli J, Munz P, Söll D (1989) In: Nasim A, Young P, Johnson BF (eds) Molecular biology of the fission yeast. Academic Press, San Diego, pp 75–96
Leupold U (1950) CR Lab Carlsberg Sér Physiol 24:381–480
Leupold U (1970) Methods Cell Physiol 4:169–177
Leupold U (1987) Curr Genet 12:543–545
Leupold U (1991) Curr Genet (in press)
Leupold U, Sipiczki M, Egel R (1991) Curr Genet (in press)
Lund PM, Hasegawa Y, Kitamura K, Shimoda C, Fukui Y, Yamamoto M (1987) Mol Gen Genet 209:627–629
McLeod M, Beach D (1988) Nature 332:509–514
Michael H, Gutz H (1987) Yeast 3:5–9
Munz P, Amstutz H, Kohli J, Leupold U (1982) Nature 300:225–231
Munz P, Wolf K, Kohli J, Leupold U (1989) In: Nasim A, Young P, Johnson BF (eds) Molecular biology of the fission yeast. Academic Press, San Diego, pp 1–30
Nadin-Davis SA, Nasim A (1988) EMBO J 7:985–993
Nadin-Davis SA, Nasim A (1990) Mol Cell Biol 10:549–560
Nadin-Davis SA, Nasim A, Beach D (1986) EMBO J 5:2963–2971
Nurse P (1985) Mol Gen Genet 198:497–502
Nurse P, Bisset Y (1981) Nature 292:558–560
Pearson D, Willis I, Hottinger H, Bell I, Kumar A, Leupold U, Söll D (1985) Mol Cell Biol 5:808–815
Perkins DD (1949) Genetics 34:607–626
Sipiczki M (1988a) Mol Gen Genet 213:529–534
Sipiczki M (1988b) Acta Microbiol Hung 35:200
Sipiczki M, Kucsera J, Dobó E (1985a) Curr Genet 9:263–272
Sipiczki M, Heyer W-D, Kohli J (1985b) Curr Microbiol 12:169–174
Thuriaux P, Sipiczki M, Fantes PA (1980a) J Gen Microbiol 116:525–528
Thuriaux P, Minet M, Munz P, Ahmad A, Zbären D, Leupold U (1980b) Curr Genet 1:89–95
Watanabe Y, Iino Y, Furuhata K, Shimoda C, Yamamoto M (1988) EMBO J 7:761–767
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Leupold, U., Sipiczki, M. Sterile UGA nonsense mutants of fission yeast. Curr Genet 20, 67–73 (1991). https://doi.org/10.1007/BF00312767
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DOI: https://doi.org/10.1007/BF00312767