Abstract
MRS6 is a newly-identified gene in the yeast Saccharomyces cerevisiae. Its product Mrs6p shows significant homology to the mammalian GDP dissociation inhibitor (GDI) of Rab/Ypt-type small G proteins and to the human choroideraemia protein (CHM), the component A of Rab-specific GGTase II. The interaction of Mrs6p with G proteins is indicated by our observation that the MRS6 gene suppresses the effect of a temperature-sensitive ypt1 mutation. Disruption of the MRS6 gene is lethal to haploid yeast cells. This is consistent with the notion that Mrs6p is interacting with Rab/Ypt-type small G proteins, which are known to have essential functions in vesicular transport. Unexpeciedly, the MRS6 gene product also affects mitochondrial functions as revealed by the facts that highcopy numbers of MRS6 (1) suppress the pet - phenotype of mrs2-1 mutant strains and (2) cause a weak pet - phenotype in wild-type strains. We conclude from these results that the MRS6 gene product has a vital function in connection with Rab/Ypt-type proteins in the cytoplasm and, in addition, affects mitochondrial functions.
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Communicated by L.A. Grivell
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Ragnini, A., Teply, R., Waldherr, M. et al. The yeast protein Mrs6p, a homologue of the rabGDI and human choroideraemia proteins, affects cytoplasmic and mitochondrial functions. Curr Genet 26, 308–314 (1994). https://doi.org/10.1007/BF00310494
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DOI: https://doi.org/10.1007/BF00310494