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The effect of chlorpromazine and tetracaine on the rapid axonal transport of neurosecretory material in the hypothalamo-neurohypophysial system of the rat

A biochemical and ultrastructural study

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Summary

The effects of chlorpromazine (cpz) and tetracaine (tc) on the rapid axonal transport of neurosecretory material (NSM) in the hypothalamo-neurohypophysial system was investigated. Following subarachnoidal injection of these drugs, the incorporation of (35S) cysteine into proteins of the supraoptic nucleus was slightly depressed. The protein-bound radioactivity in the posterior pituitary was markedly lowered in experimental rats which indicates a partial blockage of the rapid axonal transport of NSM along the hypothalamoneurohypophysial tract. Both cpz and tc induced an increase in the number of mitochondria and profiles of granular endoplasmic reticulum. The axons in the infundibulum and neurohypophysis were enlarged by dammed organelles, indicating a blockage of axonal transport. There was an increased number of microvesicles, often arranged in a crystalloid pattern, in the terminals. The number and distribution of neurotubuli and neurofilaments were not changed. A possible stimulatory effect of cpz on the release of NSM from the neural lobe is assumed Possible mechanisms for the action of mitotic inhibitors, transquilizers and local anesthetics are discussed.

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Authors and Affiliations

  1. Department of Zoophysiology and the Institute of Neurobiology, University of Göteborg, Göteborg, Sweden

    A. Edström, H.-A. Hansson & A. Norström

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  1. A. Edström
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  2. H.-A. Hansson
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  3. A. Norström
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The present work was supported by grants from “Svenska Livforsäkringsbolags Fond”, “H. Hiertas stiftelse”, “Magnus Bergwalls stiftelse”, The Swedish Medical Research Council No. B73-12X-2543-05B, “Statens naturvetenskapliga forskningsråd No. 2535-8” and from the Medical Faculty, University of Göteborg. We are indebted to Mrs. Margareta Andersson, Mrs. Wally Holmberg, Mrs. Elisabeth Norström and Mrs. Ulla Svedin for excellent technical assistance, and to Miss Gull Grönstedt for careful secretarial work.

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Edström, A., Hansson, HA. & Norström, A. The effect of chlorpromazine and tetracaine on the rapid axonal transport of neurosecretory material in the hypothalamo-neurohypophysial system of the rat. Z.Zellforsch 143, 71–91 (1973). https://doi.org/10.1007/BF00307452

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