Summary
Scanning electron microscopy of the third ventricle of sheep demonstrates areas of ciliated ependymal cells at the dorsal and middle third. The cilia of the dorsal portion of the ventricle have biconcave discs that are attached to each cilium by a slender stalk. The lower third and floor of the ventricular wall, as well as the pineal recess, are largely covered by ependymal cells that possess numerous microvilli with only a few isolated cilia scattered along cell surfaces. The infundibular recess is papillated with apical blebs of the ependymal cells that project into the lumen of the recess. Measurements of these surface elements indicate an average diameter of 0.28 μ for cilia, 0.10 μ for microvilli and 0.50 μ for the apical blebs of the infundibular recess. The functional significance of the regional differences in surface structures is discussed in relation to cerebrospinal fluid movement, ependymoabsorption and ependymosecretion.
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References
Adam, H.: Bewegung der Cerebrospinalflüssigkeit bei niederen Wirbeltieren. In: Hoff, H., Reisner, H. (eds.), Wien. Z. Nervenheilk., Suppl. 1, 70–74 (1965).
Anand Kumar, T. C.: Sexual differences in the ependyma lining the third ventricle in the area of the anterior hypothalamus of adult rhesus monkeys. Z. Zellforsch. 90, 28–36 (1968).
Barber, V. C., Boyde, A.: Scanning electron microscopic studies of cilia. Z. Zellforsch. 84, 269–284 (1968).
Bleier, R.: Structural relationship of ependymal cells and their processess within the hypothalamus. In: Knigge, K. M., Scott, D. E., Weindl, A. (eds.), Brain-endocrine interaction. Median eminence: Structure and function. Int. Symp. Munich 1971, p. 306–318. Basel-New York: S. Karger 1972.
Brightman, M. W., Palay, S. L.: The fine structure of ependyma in the brain of the rat. J. Cell Biol. 19, 415–439 (1963).
Clementi, F., Marini, D.: The surface fine structure of the walls of cerebral ventricles and of choroid plexus in cat. Z. Zellforsch. 123, 82–95 (1972).
Dalen, H., Schalpfey, W. T., Marmoon, A.: Cilia on cultured ependymal cells examined by scanning electron microscopy. Exp. Cell Res. 67, 375–379 (1971).
Fitzgerald, T. C.: Anatomy of cerebral ventricles of domestic animals. Vet. Med. 56, 38–45 (1961).
Heller, H.: Neurohypophysial hormones in the cerebrospinal fluid. In: Zirkumventrikuläre Organe und Liquor (G. Sterba, ed.), p. 235–242. Jena: Fischer 1969.
Horstmann, E.: Die Faserglia des Selachiergehirns. Z. Zellforsch. 39, 588–617 (1954).
Knigge, K. M., Scott, D. E.: Structure and function of the median eminence. Amer. J. Anat. 129, 223–244 (1970).
Knigge, K. M., Silverman, A. J.: Transport capacity of the median eminence. In: Knigge, K. M., Scott, D. E., Weindl, A. (eds.), Brain-endocrine interaction. Median eminence: Structure and function. Int. Symp. Munich 1971, p. 350–363. Basel-New York: S. Karger 1972.
Knowles, F. G. W.: Secretory cells in the ependyma. In: Heller, H., Lederis, K. (eds.), Subcellular organization and function in endocrine tissues. Memoirs of the Society of Endocrinology, No 19, p. 875–881. London-New York: Cambridge University Press 1971.
Kobayashi, H., Matsui, T.: Fine structure of the median eminence and its functional significance. In: Frontiers in neuroendocrinology, p. 3–46. New York: Oxford University Press 1969.
Kobayashi, H., Wada, M., Uemura, H., Ueck, M.: Uptake of peroxidase from the third ventricle by ependymal cells of the median eminence. Z. Zellforsch. 127, 545–551 (1972).
Löfgren, F.: The infundibular recess, a component in the hypothalamo-adenohypophyseal system. Acta morph. meerl.-scand. 3, 55–78 (1959).
Marovitz, W., Kaufman Arenberg, I., Thalmann, R.: Evaluation of preparation techniques for the scanning electron microscope. Laryngoscope (St Louis) 80, 1680–1700 (1970).
Marquet, E., Sobel, H. J., Schwarz, R., Weiss, M.: Secretion by ependymal cells of the neurohypophysis and saccus vasculosius of Polypterus ornatipinnis (Osteichthyes). J. Morph. 137, 111–130 (1972).
Matsui, T., Kobayashi, H.: Surface protrusions from the ependymal cells of the median eminence. Arch. Anat. 51, 429–436 (1968).
McFarland, W. L., Morgane, P. J., Jacobs, M. S.: Ventricular system of the brain of the dolphin, Tursiops truncatus, with comparative anatomical observations and relations to brain specializations. J. comp. Neurol. 135, 275–368 (1969).
Purkinje, J.: Über Flimmerbewegungen im Gehirn. Arch. Anat. Physiol. 3, 289–290 (1836).
Rhodin, J. A. G.: An atlas of ultrastructure, p. 1–222. Philadelphia: W. B. Saunders Co. 1963.
Schechter, J., Weiner, R.: Ultrastructural changes in the ependymal lining of the median eminence following the intraventricular administration of catecholamine. Anat. Rec. 172, 643–650 (1972).
Scott, D. E., Kozlowski, G. P., Krobisch Dudley, G.: A comparative ultrastructural analysis of the third cerebral ventricle of the North American mink (Mustela vison). Anat. Rec., in press (1972).
Scott, D. E., Krobisch Dudley, G., Gibbs, F. P., Brown, G. M.: The mammalian median eminence. A comparative and experimental model. In: Knigge, K. M., Scott, D. E., Weindl, A. (eds.), Brain-endocrine interaction. Median eminence: Structure and function. Int. Symp. Munich 1971, p. 35–49. Basel-New York: S. Karger 1972.
Scott, D. E., Paull, W. K., Krobisch Dudley, G.: A comparative scanning electron microscopic analysis of the human cerebral ventricular system. 1. The third ventricle. Z. Zellforsch. 132, 203–215 (1972).
Sharp, P. J.: Tanycyte and vascular patterns in the basal hypothalamus of Coturnix quail with reference to their possible neuroendocrine significance. Z. Zellforsch. 127, 552–569 (1972).
Siersbaek-Nielsen, K., Molholm Hansen, J.: Tyrosine and free thyroxine in cerebrospinal fluid in thyroid disease. Acta endocr. (Kbh.) 64, 126–132 (1970).
Teichmann, I., Vigh, B., Aros, B.: Histochemical studies on Gomori-positive substances II. The Gomori-positive material of a special ependymal formation (“recessus organ”) in the ventral part of the rat's third cerebral ventricle. Acta biol. Acad. Sci. hung. 17, 13–29 (1966).
Vigh, B., Aros, B., Wenger, T., Koritsánszky, S., Cegledi, G.: Ependymosecretion (ependymal neurosecretion). IV. The Gomori-positive secretion of the hypothalamic ependyma of various vertebrates and its relation to the anterior pituitary. Acta biol. Acad. Sci. hung. 13, 407–419 (1963).
Weindl, A., Joynt, R. J.: Ultrastructure of the ventricular walls. Arch. Neurol. (Chic.) 26, 420–426 (1972a).
Weindl, A., Joynt, R. J.: The median eminence as a circumventricular organ. In: Knigge, K. M., Scott, D. E., Weindl, A. (eds.), Brain-endocrine interaction. Median eminence: Structure and function. Int. Symp. Munich 1971, p. 280–297. Basel-New York: S. Karger 1972b.
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Supported by U.S.P.H.S. Grant NS 08171.
Career Development Awardee KO4 GM 70001.
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Kozlowski, G.P., Scott, D.E. & Dudley, G.K. Scanning electron microscopy of the third ventricle of sheep. Z.Zellforsch 136, 169–176 (1973). https://doi.org/10.1007/BF00307437
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DOI: https://doi.org/10.1007/BF00307437