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A scanning electron microscopic study of secondary lamellae and chloride cells of rainbow trout (Salmo gairdneri)

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Summary

Scanning electron micrographs of gill tissue from rainbow trout fixed with 50% glutaraldehyde revealed the presence of microridges on surfaces of epithelial cells of the secondary lamellae. These microridges vary in length from 1 to 7 μ, with a mean height of 0.75 μ. Calculations show that they increase the total lamellar epithelial surface area approximately 2.5 fold. Mucus secreting cells are present on the body of the filament and on secondary lamellae. Chloride cells are located primarily in the interlamellae filamental epithelium and on the basal area of lamellae. Extensions of the chloride cell epithelium are microvillous in nature and their height is only slightly greater than that of the microridges of typical lamellar epithelial cells. A reduction in number or complete absence of microvilli on chloride cells appeared to be related to degenerative changes in these cells observed in transmission electron micrographs. Non secretory interlamellae filamental epithelial cells have microridges of very attenuated lengths.

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References

  • Gray, I.E.: Comparative study of the gill area of marine fishes. Biol. Bull. 107, 219–255 (1954)

    Google Scholar 

  • Hughes, G.M.: The dimensions of fish gills in relation to their function. J. exp. Biol. 45, 177–195 (1966)

    Google Scholar 

  • Hughes, G.M., Grimstone, A.V.: The fine structure of the secondary lamellae of the gills of Gadus pollachius. Quart. J. micr. Sci. 106, 343–353 (1965)

    Google Scholar 

  • Hughes, G.M., Wright, D.E.: A comparative study of the ultrastructure of the water-blood pathway in the secondary lamellae of teleost and elasmobranch fishes—benthic forms. Z. Zellforsch. 104, 478–493 (1970)

    Google Scholar 

  • Mizuhira, V., Amakawa, T., Yamashina, S., Shirai, N., Utida, S.: Electron microscopic studies on the localization of sodium ions and sodium-potassium-activated adenosinetriphosphatase in chloride cells of eel gills. Exp. Cell Res. 59, 346–348 (1970)

    Google Scholar 

  • Muir, B.S.: Gill dimensions as a function of fish size. J. Fish. Res. Bd. Canada 26, 165–170 (1969)

    Google Scholar 

  • Muir, B.S., Hughes, G.M.: Gill dimensions for three species of tunny. J. exp. Biol. 51, 271–285 (1969)

    Google Scholar 

  • Negus, V.E.: The function of mucus. Acta oto-laryng. (Stockh.) 56, 204–214 (1963)

    Google Scholar 

  • Newstead, J.D.: Fine structure of the respiratory lamellae of teleostean gills. Z. Zellforsch. 79, 396–428 (1967)

    Google Scholar 

  • Newstead, J.D.: Observations on the relationship between “chloride-type” and “pseudobranch-type” cells in the gills of a fish Oligocottus maculosus. Z. Zellforsch. 116, 1–6 (1971)

    Google Scholar 

  • Petrik, P.: The demonstration of chloride ions in the “chloride cells” of the gills of eels (Anguilla anguilla L.) adapted to sea water. Z. Zellforsch. 92, 422–427 (1968)

    Google Scholar 

  • Reynolds, E.S.: The use of lead citrate of high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)

    Google Scholar 

  • Ritch, R., Philpott, C.W.: Repeating particles associated with an electrolyte-transport membrane. Exp. Cell Res. 55, 17–24 (1969)

    Google Scholar 

  • Shirai, N., Utida, S.: Development and degeneration of the chloride cell during seawater and freshwater adaptation of the Japanese eel Anguilla japonica. Z. Zellforsch. 103, 247–264 (1970)

    Google Scholar 

  • Spurr, A.R.: A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–43 (1969)

    Google Scholar 

  • Straus, L.P.: A study of the fine structure of the so-called chloride cell in the gill of the guppy Lebistes reticulatus P. Physiol. Zool. 36, 183–198 (1963)

    Google Scholar 

  • Threadgold, L.T., Houston, A.H.: An electronmicroscopic study of the “chloride cell” of Salmo salar L. Exp. Cell Res. 34, 1–23 (1964)

    Google Scholar 

  • Watson, M.L.: Staining of tissue sections for electronmicroscopy with heavy metal. J. biophys. biochem. Cytol. 4, 475–478 (1958)

    Google Scholar 

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Authors and Affiliations

  1. Department of Physiology, Michigan State University, East Lansing, Michigan, U.S.A.

    Kenneth R. Olson & Paul O. Fromm

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  1. Kenneth R. Olson
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  2. Paul O. Fromm
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This research was supported by EPA Grant R-801034, USPHS Training Grant HL-05873, the Mich. Agr. Exp. Sta., Proj. 122 (Journal Article No. 5801), and OWRR Grant A-064.

Acknowledgements: The authors wish to express their gratitude to Mrs. J. Mack and Mr. Wm. McAffe for their technical assistance with the electron microscopes.

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Olson, K.R., Fromm, P.O. A scanning electron microscopic study of secondary lamellae and chloride cells of rainbow trout (Salmo gairdneri). Z.Zellforsch 143, 439–449 (1973). https://doi.org/10.1007/BF00306764

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  • DOI: https://doi.org/10.1007/BF00306764

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