Summary
The light-evoked response pattern and morphology of one interplexiform cell were studied in the goldfish retina by intracellular recording and staining. The membrane potential of the cell spontaneously oscillated in the dark. In response to a brief light stimulus, the membrane potential initially gave a slow transient depolarization. During maintained light, the oscillations showed a tendency to be suppressed; the response of the cell to the offset of the stimulus was not so prominent. The perikaryon of the interplexiform cell was positioned at the proximal boundary of the inner nuclear layer. The cell had two broad layers of dendrites; one was diffuse in the inner plexiform layer, the other was more sparse in the outer plexiform layer. The morphological and electrophysiological characteristics of the cell are discussed in relation to dopaminergic interplexiform cells and the light-evoked release pattern of dopamine in the teleost retina.
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References
Bauer B, Ehinger B, Aberg L (1980) 3H-dopamine release from the rabbit retina. Albrecht von Graefes Arch Ophthalmol 215:71–78
Besharse JC, Juvone PM, Pierce ME (1988) Regulation of rhythmic photoreceptor metabolism: a role for post-receptoral neurones. Prog Retinal Res 7:21–61
Boycott BB, Dowling JE, Fisher SK, Kolb H, Laties AM (1975) Interplexiform cells of the mammalian retina and their comparison with catecholamine-containing retinal cells. Proc R Soc Lond [Biol] 191:353–368
Dearry A, Burnside B (1986) Dopaminergic regulation of cone retinomotor movement in isolated teleost retinas. I. Induction of cone contraction is mediated by D2 receptors. J Neurochem 46:1006–1021
Djamgoz MBA, Wagner H-J (1987) Intracellular staining of retinal neurones: applications to studies of functional organization. Prog Retinal Res 6:85–150
Djamgoz MBA, Wagner H-J (1991) Localization and function of dopamine in the adult vertebrate retina. Neurochem Int (in press)
Djamgoz MBA, Downing JEG, Wagner H-J (1987) Retinal neurones of cyprinid fish: intracellular marking with horseradish peroxidase and correlative morphological analysis. Exp Biol 46:203–216
Djamgoz MBA, Downing JEG, Wagner H-J (1989a) An intracellular horseradish peroxidase study of amacrine cells in the cyprinid fish retina. Cell Tissue Res 256:607–622
Djamgoz MBA, Kirsch M, Wagner H-J (1989b) Haloperidol suppresses light-induced spinule formation and biphasic responses of horizontal cells in fish (roach) retina. Neurosci Lett 107:12–16
Djamgoz MBA, Spadavecchia L, Usai C, Vallerga S (1990) Light-evoked response pattern variability and morphological characterization of macrine cells in goldfish retina. J Comp Neurol 301:171–190
Dowling JE (1979) A new retinal neurone — the interplexiform cell. Trends Neurosci 3:189–191
Dowling JE (1986) Dopamine: a retinal neuromodulator? Trends Neurosci 9:236–240
Dowling JE (1987) The retina. An approachable part of the brain. Belknap, Harvard University Press Cambridge
Dowling JE, Ehinger B (1975) Synaptic organization of the amine-containing interplexiform cells of the goldfish and Cebus monkey retinas. Science 188:270–273
Dowling JE, Ehinger B (1978) The interplexiform cell system. I. Synapses of the dopaminergic neurons of the goldfish retina. Proc R Soc Lond [Biol] 201:7–26
Dowling JE, Watling KJ (1981) Dopaminergic mechanisms in the teleost retina. II. Factors affecting the accumulation of cyclic AMP in pieces of intact carp retina. J Neurochem 36:569–579
Ehinger B (1982) Neurotransmitter systems in the retina. Retina 2:305–321
Famiglietti EV Jr, Kaneko A, Tachibana M (1977) Neuronal architecture of ON and OFF pathways to ganglion cells in carp retina. Science 198:1267–1268
Hashimoto Y, Abe M, Inokuchi M (1980) Identification of the interplexiform cell in the dace retina by dye injection method. Brain Res 197:331–340
Hedden WL, Dowling JE (1978) The interplexiform cell system II. Effects of dopamine on goldfish retinal neurones. Proc R Soc Lond [Biol] 201:27–55
Hosokawa Y, Naka K-I (1985) Spontaneous membrane fluctuations in catfish type-N cells. Vision Res 25:539–542
Kalloniatis M, Marc RE (1990) Interplexiform cells in the goldfish retina: a combined Golgi and autoradiographic study. J Comp Neurol 297:340–358
Kirsch M, Wagner H-J (1989) Release pattern of endogenous dopamine in teleost retinae during light adaptation and pharmacological stimulation. Vision Res 29:147–154
Kirsch M, Wagner H-J, Djamgoz MBA (1991) Dopamine and plasticity of horizontal cell function in the retina: regulation of a spectral mechanism through D1 receptors. Vision Res (in press)
Knapp AG, Dowling JE (1987)_Dopamine enhances excitatory amino acid-gated conductances in retinal horizontal cells. Nature 325:437–439
Kolb H, West RW (1977) Synaptic connections of the interplexiform cell in the retina of the cat. J Neurocytol 6:155–170
Kramer SG (1971) Dopamine: a retinal neurotransmitter. I. Retinal uptake, storage, light-stimulated release of 3H-dopamine in vivo. Invest Ophthalmol 10:438–452
Linberg KA, Fisher SK (1986) An ultrastructural study of interplexiform cell synapses in the human retina. J Comp Neurol 243:561–576
Maguire G, Lukasiewicz P, Werblin F (1988) Electrical, morphological and pharmacological properties of interplexiform cells in the tiger salamander retina. Invest Ophthalmol Visual Sci 29:224
Maguire G, Lukasiewicz P, Werblin F (1990) Synaptic and voltagegated currents in interplexiform cells of the tiger salamander retina. J Gen Physiol 95:755–772
Mangel SC, Dowling JE (1985) Responsiveness and receptive field size of carp horizontal cells are reduced by prolonged darkness and dopamine. Science 229:1107–1109
Mangel SC, Dowling JE (1987) The interplexiform — horizontal cell system of the fish retina: effects of dopamine, light stimulation and time in the dark. Proc R Soc Lond [Biol] 231:91–121
Marc RE, Liu W-LS (1984) Horizontal cell synapses onto glycine-accumulating interplexiform cells. Nature 312:266–269
Negishi K, Drujan B (1979) Effects of catecholamines and related compounds on horizontal cells in the fish retina. J Neurosci Res 4:311–334
Negishi K, Teranishi T, Kato S (1981) Density of retinal dopaminergic cells and indoleamine-accumulating cells in different-sized carp. Acta Histochem Cytochem 14:596–606
Oyster CW, Takahashi ES (1977) Interplexiform cells in rabbit retina. Proc R Soc Lond [Biol] 197:477–484
Reading HW (1983) Dopaminergic receptors in bovine retina and their interaction with thyrotropin-releasing hormone. J Neurochem 41:1587–1595
Sarthy V, Lam DM-K (1979) The uptake and release of [3H]dopamine in the goldfish retina. J Neurochem 32:1269–1277
Teranishi T, Negishi K (1986) Dendritic morphology of dopaminergic cells revealed by intracellular injection of Lucifer yellow in fixed carp retina. Brain Res 370:196–199
Teranishi T, Negishi K, Kato S (1983) Dopamine modulates S-potential amplitude and dye-coupling between external horizontal cells in carp retina. Nature 301:243–246
Teranishi T, Negishi K, Kato S (1987) Functional and morphological correlates of amacrine cells in carp retina. Neurosci 20:935–950
Wagner H-J, Wulle I (1990) Dopaminergic interplexiform cells contact photoreceptor terminals in catfish retina. Cell Tissue Res 261:359–365
Yasui S, Yamada M, Djamgoz MBA (1990) Dopamine and 2-amino-4-phosphono-butyrate differentially modify spectral responses of H1 horizontal cells in carp retina. Exp Brain Res 83:79–84
Yazulla S, Zucker C (1988) Synaptic organization of dopaminergic interplexiform cells in the goldfish retina. Visual Neurosci 1:13–29
Zucker C, Dowling JE (1987) Centrifugal fibres on dopaminergic interplexiform cells in the teleost retina. Nature 330:166–168
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Djamgoz, M.B.A., Usai, C. & Vallerga, S. An interplexiform cell in the goldfish retina: light-evked response pattern and intracellular staining with horseradish peroxidase. Cell Tissue Res 264, 111–116 (1991). https://doi.org/10.1007/BF00305728
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DOI: https://doi.org/10.1007/BF00305728