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Mediation of serotonin-induced hyperventilation via 5-HT3-receptor in European eel Anguilla anguilla

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Abstract

The effects of serotonin (5-hydroxytryptamine) on ventilation were investigated by continuous measurements of intrabuccal pressure in unrestrained eel. Intravenous administration of 5-hydroxytryptamine (30 μg·kg-1) caused a large increase in ventilatory frequency (+100%) and amplitude (+140%). The 5-hydroxytryptamine-induced hyperventilation was blocked by the 5-HT3-receptor antagonists metoclopramide (1.0 mg·kg-1) or MDL72222 (1.0 mg·kg-1), and was insensitive to the 5-HT1/2-receptor antagonist methysergide (3.0 mg·kg-1) and to the 5-HT4-receptor antagonist DAU 6285 CL (3.0 mg·kg-1). The hyperventilatory response to 5-hydroxytryptamine could be mimicked by the 5-HT3 receptor agonist 1-phenylbiguanide (300 μg·kg-1). These results strongly implicate the 5-HT3-receptor as the mediator of the 5-hydroxytryptamine-induced hyperventilation in eel.

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Abbreviations

a.u. :

arbitrary units

5-HT :

5-hydroxytryptamine

SEM :

standard error of mean

VA :

ventilatory amplitude

VF :

ventilatory frequency

RBI :

1-phenylbiguanide

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Authors and Affiliations

  1. Laboratoire de Physiologie Animale, Faculté des Sciences et Techniques, 6 Avenue Victor Le Gorgeu, F-29287, Brest, France

    J. -J. Janvier, M. Peyraud-Waïtzenegger & P. Soulier

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  1. J. -J. Janvier
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  2. M. Peyraud-Waïtzenegger
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  3. P. Soulier
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Communicated by H. Langer

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Janvier, J.J., Peyraud-Waïtzenegger, M. & Soulier, P. Mediation of serotonin-induced hyperventilation via 5-HT3-receptor in European eel Anguilla anguilla . J Comp Physiol B 165, 640–646 (1996). https://doi.org/10.1007/BF00301132

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