Summary
Histogenesis and histochemistry of submandibular and sublingual glands of mouse embryo were studied both during normal development and in vitro. In normal conditions secretory activity becomes detectable by histochemical methods at the 16th day of gestation as intracellular material. From the start, secretion shows a positive reaction to the PAS and Hale's method, and stains with alcian blue. At the 17th day differences may be shown between the two glands in the morphological features of the secretion, in the intensity of the above said histochemical reactions, and in the behaviour towards toluidine blue, which after sulfation gives metachromatic staining in the sublingual, but not in the submandibular gland. This indicates that the polysaccharidic component of the secretion has different characters in the two glands.
Cultures in vitro were made of glands at the 13th day of gestation, on a clot of chicken plasma and embryo extract. At explantation time, they are just beginning their differentiation, and both consist of an epithelial cord and a solid bud, contained in a common mesenchymal capsule. The ramification process goes on at a slower rate in vitro than in vivo, but secretion appears at the same total age. The glands developed in vitro for 10 days and their histochemical features appeared to be comparable to those which were observed in normal developmental conditions.
Riassunto
E' stata studiata l'istogenesi e l'istochimica della ghiandola sottomandibolare e sottolinguale di embrione di topo, in condizioni di sviluppo normale e in cultura organotipica in vitro. In condizioni normali l'attività secretrice diviene dimostrabile con mezzi istochimici al 16∘ giorno di gestazione, come materiale intracellulare. La secrezione presenta fino dall'inizio reazione positiva ai metodi del PAS e di Hale, ed è colorabile col blu alcian. Al 17∘ giorno compaiono fra le due ghiandole differenze nell'aspetto morfologico del secreto, nell'intensità delle suddette reazioni istochimiche, e nel comportamento rispetto al blu di toluidina che dopo solfatazione dà colorazione metacromatica nella sottolinguale, ma non nella sottomandibolare. Ciò indica che il componente polisaccaridico del secreto ha caratteristiche un po' diverse nelle due ghiandole.
Sono state eseguite culture in vitro di ghiandole al 13∘ giorno di gestazione, su un coagulo di plasma e di estratto embrionale di pollo. Al momento dell'espianto, esse sono all'inizio della loro differenziazione, e sono formate ciascuna da un cordoncino epiteliale e da una gemma solida, entro una comune capsula mesenchimale. Il processo di ramificazione si svolge più lentamente che in vivo, ma la secrezione compare alla stessa età totale. Le ghiandole si sono sviluppate in vitro per 10 giorni e le loro caratteristiche istochimiche sono apparse eguali a quelle che si osservano in condizioni normali di sviluppo.
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Lavoro eseguito coi mezzi forniti da contratti di ricerca dell'U.S.A.E.C. (NYO-3355-10) e dell'Euratom (043-65-1 BIO I) e con un contributo del C.N.R.
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Di Giovine Vecchione, L. Osservazioni sulla secrezione delle ghiandole sottomandibolare e sottolinguale di embrione di topo, in condizioni normali e in cultura in vitro . Histochemie 9, 203–216 (1967). https://doi.org/10.1007/BF00294391
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DOI: https://doi.org/10.1007/BF00294391