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Genetical and structural analysis of a group of λilv and λrho transducing phages

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Summary

Eight λilvC transducing phages generated from E. coli K12 secondary site lysogens have been analysed genetically and physically. Two of them carry, in addition, the rho gene and its promotor region, but not the cya gene. The ilvO603 mutation has been located between ilvG and ilvE. Electrophoretic analysis of the proteins synthesized by these phages in a system of UV irradiated cells allowed us to assign molecular weights of 55000 and 66000 daltons to the ilvC and the ilvD gene products, respectively, and to show that an ilvG-encoded polypeptide of 60000 daltons is made from an ilvO - but not from an ilvO + phage. The expression of the ilvG gene is discussed in the light of the recent finding of a promoter-attenuator region lying upstream to ilvG. Finally, we have found that one of the λilv phages does not have the classical structure of a transducing phage.

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References

  • Abrescia P, Guardiola J, Foresti M, Lamberti A, Iaccarino M (1979) Thereonine deaminase: Autogenous regulator of the ilv genes in Escherichia coli K-12. Mol Gen Genet 171:261–275

    Google Scholar 

  • Arfin ST, Ratzkin B, Umbarger HE (1969) The metabolism of valine and isoleucine in Escherichia coli. XVII The role of induction in the derepression of acetohydroxy acid isomeroreductase. Biochem Biophys Res Commun 37:902–908

    Google Scholar 

  • Bachmann BJ, Low KB (1980) Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev 44:1–56

    Google Scholar 

  • Baez M, Patin DW, Calhoun DH (1979) Deletion mapping of the ilvGEDAC genes of Escherichia coli K-12. Mol Gen Genet 169:289–297

    Google Scholar 

  • Berg CM, Shaw KJ, Berg DE (1980) The ilvG gene is expressed in Escherichia coli K-12. Gene 12:165–170

    Google Scholar 

  • Bonner WM, Laskey RA (1974) A film detection method for tritiumlabelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem 46:83–88

    Google Scholar 

  • Calhoun DH, Rimerman RA, Hatfield GW (1973) Threonine deaminase from Escherichia coli. I. Purification and properties. J Biol Chem 248:3511–3516

    Google Scholar 

  • Campbell A (1971) Genetic structure. In: Hershey AD (ed) The bacteriophage λ. Cold Spring Harbor Laboratory, New York, p 13

    Google Scholar 

  • Childs GJ, Ohtsubo H, Ohtsubo E, Sonnenberg F, Freundlich M (1977) Restriction endonuclease mapping of the Escherichia coli K-12 chromosome in the vicinity of the ilv genes. J Mol Biol 117:175–193

    Google Scholar 

  • Cohen BM, Jones EW (1976) New map location of ilvO in Escherichia coli. Genetics 83:201–225

    Google Scholar 

  • Das A, Court, D., Adhya S (1976) Isolation and characterization of conditional lethal mutants of Escherichia coli defective in transcription termination factor rho. Proc Natl Acad Sci USA 73:1959–1963

    Google Scholar 

  • Davis RM, Simon M, Davidson N (1971) Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. In: Grossman L and Moldave K (eds) Methods in enzymology, vol 21. Academic Press, New-York, p 413

    Google Scholar 

  • De Felice M, Guardiola J, Malorni MC, Klopotowski T, Iaccarino M (1974) Regulation of the pool size of valine in Escherichia coli K-12. J Bacteriol 120:1058–1067

    Google Scholar 

  • De Felice M, Squires C, Levinthal M, Guardiola, J., Lamberti A, Iaccarino M (1977) Growth inhibition of Escherichia coli K-12 by valine: a consequence of a regulatory pattern. Mol Gen Genet. 156:1–7

    Google Scholar 

  • De Felice M, Levinthal M, Iaccarino M, Guardiola J (1979) Growth inhibition as a consequence of antagnonism between related amino acids: effects of valine in Escherichia coli K-12. Microbiol Rev 43:42–58

    Google Scholar 

  • Enquist L, Tierneier D, Leder P, Weisberg R, Stenberg N (1976) Safer derivatives of bacteriophage λgt, λC for use in cloning of recombinant DNA molecules. Nature 259:597–598

    Google Scholar 

  • Favre R, Wiater A, Puppo S, Iaccarino M, Noelle R, Freundlich M (1976) Expression of a valine-resistant acetolactate, synthase activity mediated by the ilvO and ilvG genes of Escherichia coli K-12. Mol Gen Genet 143:243–252

    Google Scholar 

  • Franklin NC, Yanofsky C (1976) The N protein of λ: evidence bearing on transcription termination polarity and the alteration of Escherichia coli RNA polymerase. In Losick R and Chamberlin, M (eds) RNA polymerase. Cold Spring Harbor Laboratory, New York, p 693

    Google Scholar 

  • Hopkins AS, Murray NE, Brammar WJ (1976) Characterization of λtrp transducing bacteriophages made in vitro. J Mol Biol 107:549–569

    Google Scholar 

  • Iaccarino M, Guardiola J, De Felice M, Favre R (1978) Regulation of isoleucine and valine biosynthesis. In Current topics in cellular regulation 14: 29–73

  • Imai M, Shigesada K (1978) Studies on the altered rho factor in nitA mutants of Escherichia coli defective in transcription termination. I. Characterization and quantitative determination of rho in cell extracts. J Mol Biol 120:451–466

    Google Scholar 

  • Inoko H, Shigesada K, Imai K (1977) Isolation and characterization of conditional-lethal rho mutants of Escherichia coli. Proc Natl Acad Sci USA 74:1162–1166

    Google Scholar 

  • Jørgensen P, Collins J, Fiil N, Von Meyenburg K (1978) A ribosomal RNA gene, rrnC, of Escherichia coli, mapped by specialized transducing λdilv and λdrbs phages. Mol Gen Genet 163:223–228

    Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Google Scholar 

  • Lawther RP, Nichols B, Zurawski G, Hatfield GW (1979) The nucleotide sequence preceding and including the beginning of the ilvE gene of the ilvGEDA operon of Escherichia coli K-12. Nucl Acids Res 7:2289–2301

    Google Scholar 

  • Lawther RP, Hatfield GW (1980) Multivalent translational control of transcription termination at attenuator of ilvGEDA operon of Escherichia coli K-12. Proc Natl Acad Sci USA 77:1862–1866

    Google Scholar 

  • Lee-Peng FC, Hermodson MA, Kohlhaw GB (1979) Transaminase B from Escherichia coli: quanternary structure amino-terminal sequence, substrate specificity and absence of a separate, valine α-ketoglutarate activity. J Bacteriol 139:339–345

    Google Scholar 

  • Lo Schiavo F, Favre R, Kasai T, Cascino A, Guardiola J, Caro L, Iaccarino M (1975) The Φ80λdilv phage and its use to detect messenger RNA related to isoleucine-valine metabolsim. J Mol Biol 99:353–368

    Google Scholar 

  • Marsh NJ, Duggan DE (1972) Ordering of mutant sizes in the isoleucine-valine genes of Escherichia coli by use of merogenotes derived from F-14: a new procedure for fine-structure mapping. J Bacteriol 109:730–740

    Google Scholar 

  • McCorkle GM, Leathers TD, Umbarger HE (1978) Physical organization of the ilvEDAC genes of Escherichia coli strain K-12. Proc Natl Acad Sci USA 75:89–93

    Google Scholar 

  • Miller JH (1972) In: Experiments in molecular genetics. Cold Spring Harbor Laboratory, New York

    Google Scholar 

  • Newman TC, Levinthal M (1980) A new map location for the ilvB locus of Escherichia coli K-12. Genetics 96:59–77

    Google Scholar 

  • Nargang FE, Subrahmanyam CS, Umbarger HE (1980) Nucleotide sequence of the attenuator region of the ilvGEDA operon of Escherichia coli. Proc Natl Acad Sci USA 77:1823–1827

    Google Scholar 

  • Oppenheim AB, Katzir N, Oppenheim A (1977) Regulation of protein synthesis in bacteriophage λ. Restoration of gene expression in λN - strains by mutation in the cro gene. Virologie 79:405–425

    Google Scholar 

  • Patin DW, Calhoun DM (1979) Mapping of ilvO loci of Escherichia coli K-12 with bacteriophage λdilv. J Bacteriol 137:1234–1242

    Google Scholar 

  • Ptashne M (1967) Isolation of the λ repressor. Proc Natl Acad Sci USA 57:306–313

    Google Scholar 

  • Roberts JW (1969) Termination factor for RNA synthesis. Nature 224:1169–1174

    Google Scholar 

  • Schrenk WJ, Weisberg RA (1975) A simple method for making new transducing lines of coliphage λ. Mol Gen Genet 137:101–107

    Google Scholar 

  • Shimada K, Weisberg RA, Gottesman ME (1973) Prophage lambda at unusual chromosomal locations. II. Mutations induced by bacteriophage lambda in Escherichia coli K-12. J Mol Biol 80:297–314

    Google Scholar 

  • Shimada K, Weisberg RA, Gottesman ME (1975) Prophage lambda at unusual chromosomal locations. III. The components of the secondary attachment sites. J Mol Biol 93:415–429

    Google Scholar 

  • Silver L, Chandler M, Boy de la Tour E, Caro L (1977) Origin and direction of replication of the drug resistance plasmid R100-1 and of a resistance transfer factor derivative in synchronized cultures. J Bacteriol 131:929–942

    Google Scholar 

  • Smith J, Smith F, Umbarger H (1979) Mutation affecting the formation of acetohydroxyacid synthase II in Escherichia coli K-12. Mol Gen Genet 169:299–314

    Google Scholar 

  • Springer M, Graffe M, Hennecke H (1977) Specialized transducing phage for the initiation factor IF3 gene in E. coli. Proc Natl Acad Sci USA 74:3970–3974

    Google Scholar 

  • Steitz JA (1979) RNA-RNA interaction during polypeptide chain initation. In: Chambliss G, Graven GR, Davies J, Davis K, Kahan L, Nomura M (eds) Ribosomes: Structure, function and genetics. University Park Pres, Baltimore, p 479

    Google Scholar 

  • Subrahmanyam CS, McCorkle GM, Umbarger HE (1980a) Physical location of the ilvO determinant in Escherichia coli K-12 deoxyribonucleic acid. J Bacteriol 142:547–555

    Google Scholar 

  • Subrahmanyam CS, Noti JD, Umbarger HE (1980b) Regulation of ilvEDA expression occurs upstream of ilvG in Escherichia coli: Additional evidence for an ilvGEDA operon. J Bacteriol 144:279–290

    Google Scholar 

  • Sutcliffe JG (1978) pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Nucl Acids Res 5:2721–2728

    Google Scholar 

  • Szybalski EH, Szybalski W (1979) A comprehensive molecular map of bacteriophage lambda. Gene 7:217–270

    Google Scholar 

  • Thomas M, Cameron JR, Davis RW (1974) Viable molecular hybrids of bacteriophage lambda and eucaryotic DNA. Proc Natl Acad Sci USA 71:4579–4583

    Google Scholar 

  • Thomas M, Davis RW (1975) Studies on the cleavage map of bacteriophage lambda with EcoRI restriction endonuclease. J Mol Biol 91:315–328

    Google Scholar 

  • Umbarger HE (1978) Amino acid biosynthesis and its regulation. Ann Rev Biochem 47:533–606

    Google Scholar 

  • Watson MD, Wild J, Umbarger HE (1979) Positive control of ilvC expression in Escherichia coli K-12; identification and mapping of the ilvY regulatory gene. J. Bacteriol 139:1014–1020

    Google Scholar 

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Authors and Affiliations

  1. Institut de Biologie Physico-Chimique, 13, rue Pierre et Marie Curie, F-75005, Paris, France

    Marc Uzan & Renée Favre

  2. Département de Biologie Moléculaire, Université de Genève, 30, Quai Ernest-Ansermet, CH-1211, Genève 4, Switzerland

    Edith Gallay & Lucien Caro

Authors
  1. Marc Uzan
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  2. Renée Favre
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  3. Edith Gallay
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  4. Lucien Caro
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Communicated by W. Arber

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Uzan, M., Favre, R., Gallay, E. et al. Genetical and structural analysis of a group of λilv and λrho transducing phages. Molec. Gen. Genet. 182, 462–470 (1981). https://doi.org/10.1007/BF00293936

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  • DOI: https://doi.org/10.1007/BF00293936

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