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Cloning, sequencing and characterization of the Saccharomyces cerevisiae URA7 gene encoding CTP synthetase

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Summary

The URA7 gene of Saccharomyces cerevisiae encodes CTP synthetase (EC 6.3.4.2) which catalyses the conversion of uridine 5′-triphosphate to cytidine 5′-triphosphate, the last step of the pyrimidine biosynthetic pathway. We have cloned and sequenced the URA 7 gene. The coding region is 1710 by long and the deduced protein sequence shows a strong degree of homology with bacterial and human CTP synthetases. Gene disruption shows that URA7 is not an essential gene: the level of the intracellular CTP pool is roughly the same in the deleted and the wild-type strains, suggesting that an alternative pathway for CTP synthesis exists in yeast. This could involve either a divergent duplicated gene or a different route beginning with the amination of uridine mono- or diphosphate.

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Authors and Affiliations

  1. Centre de Génétique Moléculaire du C.N.R.S., Laboratoire propre associé à l'Université Pierre et Marie Curie, F-91198, Gif-sur-Yvette Cedex, France

    Odile Ozier-Kalogeropoulos, Jocelyne Collin & François Lacroute

  2. I.B.M.C. du C.N.R.S., 15 rue R. Descartes, F-67084, Strasbourg Cedex, France

    Franco Fasiolo

  3. Institut de Chimie des Substances Naturelles du C.N.R.S., F-91198, Gif-sur-Yvette Cedex, France

    Marie-Therèse Adeline

Authors
  1. Odile Ozier-Kalogeropoulos
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  2. Franco Fasiolo
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  3. Marie-Therèse Adeline
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  4. Jocelyne Collin
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  5. François Lacroute
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Communicated by C.P. Hollenberg

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Ozier-Kalogeropoulos, O., Fasiolo, F., Adeline, MT. et al. Cloning, sequencing and characterization of the Saccharomyces cerevisiae URA7 gene encoding CTP synthetase. Molec. Gen. Genet. 231, 7–16 (1991). https://doi.org/10.1007/BF00293815

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