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Patterns of incorporation of tritiated thymidine by the dorsal polytene foot-pad nuclei of Sarcophaga bullata (Sarcophagidae: Diptera)

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Abstract

The incorporation of tritiated thymidine into the DNA of the dorsal foot-pad nuclei of Sarcophaga bullata, during pharate adult development, was studied by scintillation counting and autoradiography. Incorporation was maximal on day 4 and showed a progressive temporal decline on days 5 to 8. Autoradiographs of chromosomal arms A1, A2 and D1, from late stages of the prolonged S period, showed discontinuous incorporation. On days 7 and 8 loci which incorporated the radio-labeled precursor were almost exclusively associated with DNA granules. DNA granules were associated with 8 specific loci in chromosomal arm A1, 10 in A2, and 8 in D1. The relationship between patterns of incorporation of tritiated thymidine and DNA granules is discussed.

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Author notes

  1. Brian Roberts

    Present address: Department of Zoology, Monash University, 3168, Clayton, Victoria, Australia

Authors and Affiliations

  1. Department of Biological Sciences, Northwestern University, 60201, Evanston, Illinois, USA

    Brian Roberts, Joan M. Whitten & Lawrence I. Gilbert

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  1. Brian Roberts
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  2. Joan M. Whitten
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  3. Lawrence I. Gilbert
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Deceased June 15, 1975

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Roberts, B., Whitten, J.M. & Gilbert, L.I. Patterns of incorporation of tritiated thymidine by the dorsal polytene foot-pad nuclei of Sarcophaga bullata (Sarcophagidae: Diptera). Chromosoma 54, 127–140 (1976). https://doi.org/10.1007/BF00292835

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  • DOI: https://doi.org/10.1007/BF00292835

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