Summary
A method of genetic analysis is presented which involves digestion of DNA with a single restriction enzyme (PvuII) and hybridisation with a mixture of five probes. Four of the five probes chosen recognise hypervariable regions (HVRs) of the human genome and hence an allele-specific DNA identity pattern results. An advantage of this approach to genetic characterisation is that the complex identity patterns may be broken down into simple allelic systems of known chromosomal localization by hybridisation with the individual probes. Also different probes may be included in a combined probe designed for particular types of investigation.
Similar content being viewed by others
References
Bell JI, Estess P, St John T, Saiki R, Watling DL, Erlich HA, McDevitt HO (1985) DNA sequence and characterisation of human class II major histocompatibility complex β chains from the DR1 haplotype. Proc Natl Acad Sci USA 82:3405–3409
Cooper DN, Schmidtke J (1984) DNA restriction fragment length polymorphisms and heterozygosity in the human genome. Hum Genet 66:1–17
Gill P, Jeffreys AJ, Werrett DJ (1985) Forensic application of DNA ‘fingerprints’. Nature 318:577–579
Ginsburg D, Antin JH, Smith BR, Orkin SH, Rappeport JM (1985) Origin of cell populations after bone marrow transplantation J Clin Invest 75:596–603
Glaser T, Lewis WH, Bruns GAP, Watkins PC, Rogler CE, Shows TB, Powers VE, Willard HF, Goguen JM, Simola KOJ, Housman DE (1986) The β-subunit of follicle-stimulating hormone is deleted in patients with aniridia and Wilms' tumour, allowing a further definition of the WAGR locus. Nature 321:882–887
Gusella JF (1986) Recombinant DNA techniques in the diagnosis of inherited disorders. J Clin Invest 77:1723–1726
Higgs DR, Goodbourn SEY, Wainscoat JS, Clegg JB, Weatherall DJ (1981) Highly variable regions of DNA flank the human α globin genes. Nucleic Acids Res 9:4213–4224
Higgs DR, Wainscoat JS, Flint J, Hill AVS, Thein SL, Nicholls RD, Teal H, Ayyub H, Peto TEA, Falusi Y, Jarman AP, Clegg JB, Weatherall DJ (1986) Analysis of the human α-globin gene cluster reveals an highly informative locus. Proc Natl Acad Sci USA 83: 5165–5169
Hill AVS, Jeffreys AJ (1985) Use of minisatellite DNA probes for determination of twin zygosity at birth. Lancet II:1394–1395
Hill AVS, Nicholls RD, Thein SL, Higgs DR (1985) Recombination within the human embryonic ζ-globin locus. A common ζ-ζ chromosome produced by gene conversion of the ζ gene. Cell 42: 809–819
Jeffreys AJ, Wilson V, Thein SL (1985a) Hypervariable ‘minisatellite’ regions in human DNA. Nature 314:67–73
Jeffreys AJ, Wilson V, Thein SL (1985b) Individual-specific ‘fingerprints’ of human DNA. Nature 316:76–79
Jeffreys AJ, Brookfield JFY, Semeonoff R (1985c) Positive. identification of an immigration test case using human DNA fingerprints. Nature 317:818–819
Knowlton RG, Brown VA, Braman JC, Barker D, Schumm JW, Murray C, Takvorian T, Ritz J, Doris-Keller H (1986) Use of highly polymorphic DNA probes for genotypic analysis following bone marrow transplantation. Blood 68:378–385
Lan YF, Huang JC, Dozy AM, Kan YW (1984) Rapid screening test for antenatal sex determination. Lancet I:14–16
Old JM, Higgs DR (1982) Gene analysis. In: Weatherall DJ (ed) The thalassaemias methods in haematology. Churchill Livingstone, London Edinburgh
Old JM, Wainscoat JS (1983) A new DNA polymorphism in the β-globin gene cluster can be used for antenatal diagnosis of β-thalassaemia. Br J Haematol 53:337–341
Proudfoot NJ, Gil A, Maniatis TM (1982) The structure of the human zeta-globin gene and a closely linked, nearly identical pseudogene. Cell 31:553–563
Reeders ST, Breuning MH, Davies KE, Nicholls RD, Jarman AP, Higgs DR, Pearson PL, Weatherall DJ (1985) A highly polymorphic DNA marker linked to adult polycystic kidney disease on chromosome 16 Nature 317:542–544
Schumm JW, Knowlton RG, Braman JC, Barker D, Vovis G, Akots G, Brown V, Gravius T, Helms C, Hsiao K, Rediker K, Thurston J, Botstein D, Doris-Keller H (1985) Detection of more than 500 single copy RFLP's by random screening. (8th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 40:739
Smouse PE, Chakraborty R (1986) The use of restriction fragment length polymorphisms in paternity analysis. Am J Hum Genet 38: 918–936
Thein SL, Oscier DG, Flint J, Wainscoat JS (1986) Ha-ras hypervariable alleles in myelodysplasia. Nature 321:84–85
Vergnaud G, Kaplan L, Weissenbach J, Dumez Y, Berger R, Tiollais P, Guelloen G (1984) Rapid and early determination, of sex using trophoblast biopsy specimens and Y chromosome specific DNA probes. Br Med J 284:73–76
Wainscoat JS, Kulozik AE, Ramsay M, Falusi AG, Weatherall DJ (1986) A Taq 1γ-globin DNA polymorphism: an African-specific marker. Hum Genet 74:90–92
Wyman AR, White R (1980) A highly polymorphic locus in human DNA. Proc Natl Acad Sci USA 77:6754–6758
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wainscoat, J.S., Pilkington, S., Peto, T.E.A. et al. Allele-specific DNA identity patterns. Hum Genet 75, 384–387 (1987). https://doi.org/10.1007/BF00284114
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00284114