Abstract
Pyrimidine biosynthesis was investigated in Pseudomonas cepacia ATCC 17759. The presence of the de novo pyrimidine biosynthetic pathway enzyme activities was confirmed in this strain. Following transposon mutagenesis of the wild-type cells, a mutant strain deficient for orotidine 5′-monophosphate decarboxylase activity (pyrF) was isolated. Uracil, cytosine or uridine supported the growth of this mutant. Uracil addition to minimal medium cultures of the wild-type strain diminished the levels of the de novo pyrimidine biosynthetic enzyme activities, while pyrimidine limitation of the mutant cells increased those de novo enzyme activities measured. It was concluded that regulation of pyrimidine biosynthesis at the lelel of enzyme synthesis in P. cepacia was present. Aspartate transcarbamoylase activity was found to be regulated in the wild-type cells. Its activity was shown to be controlled in vitro by inorganic pyrophosphate, adenosine 5′-triphosphate and uridine 5′-phosphate.
References
Adair LB, Jones ME (1972) Purification and characteristics of aspartate trancarbamoylase from Pseudomonas fluorescens. J Biol Chem 247: 2308–2315
Beckwith JR, Pardee AB, Austrian R, Jacob F (1962) Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli. J Mol Biol 5: 618–634
Beringer JE, Benyon JL, Buchanan-Wollaston AV, Johnston AWB (1978) Tranfer of the drug-resistance transposon Tn5 to Rhizobium. Nature 276: 633–634
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye-binding. Anal Biochem 72: 248–254
Condon S, Collins JK, O'Donnovan GA (1976) Regulation of arginine and pyrimidine biosynthesis in Pseudomonas putida. J Gen Microbiol 92: 375–383
Gantotti BV, Kindle KL, Beer SV (1981) Transfer of the drug-resistance transposon Tn5 to Erwinia herbicola and the induction of insertion mutations. Curr Microbiol 6: 377–381
Isaac JH, Holloway BW (1968) Control of pyrimidine biosynthesis in Pseudomonas aeruginosa. J Bacteriol 96: 1732–1741
Kelln RA, Kinahan JJ, Foltermann KF, O'Donovan GA (1975) Pyrimidine biosynthetic enzymes of Salmonella typhimurium, repressed specifically by growth in the presence of cytidine. J Bacteriol 124: 764–774
Monticello DJ, Bakker D, Schell M, Finnerty WR (1985) Plasmid-borne Tn5 insertion mutation resulting in accumulation of gentisate from salicylate. Appl Environ Microbiol 49: 761–764
Neumann J, Jones ME (1964) End-product inhibition of aspartate transcarbamylase in various species. Arch Biochem Biophys 104: 438–447
O'Donovan GA, Neuhard J (1970) Pyrimidine metabolism in micro-organisms. Bacteriol Rev 34: 278–343
Prescott LM, Jones ME (1969) Modified methods for the determination of carbamyl aspartate. Anal Biochem 32: 408–419
Schwartz M, Neuhard J (1975) Control of expression of the pyr genes in Salmonella typhimurium: effects of variations in uridine and cytidine nucleotide pools. J Bacteriol 121: 814–822
Stanier RY (1947) Simultaneous adaption: a new technique for the study of metabolic pathways. J Bacteriol 54: 339–348
Stanier RY, Palleroni NJ, Doudoroff M (1966) The aerobic pseudomonads: a taxonomic study. J Gen Microbiol 43: 159–271
West TP (1989) Isolation and characterization of thymidylate synthetase mutants of Xanthomonas maltophilia. Arch Microbiol 151: 220–222
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West, T.P., Chu, Cp. Regulation of pyrimidine biosynthesis in Pseudomonas cepacia . Arch. Microbiol. 154, 407–409 (1990). https://doi.org/10.1007/BF00276539
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DOI: https://doi.org/10.1007/BF00276539