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Regeneration of plants from mesophyll protoplasts of the wild crucifer Eruca sativa Lam.

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Abstract

Protoplasts isolated from mesophyll cells of Eruca sativa Lam., cultured on suitable medium, underwent sustained cell divisions to form calli. The plating efficiency was found to be 0.4%. The protoplast-derived calli subsequently produced plantlets through organogenesis (15.71%) and somatic embryogenesis (11.25%). Regenerated plants exhibited normal appearance. These results indicate potential to introgress desirable traits from this wild crucifer into important oilseed and cole Brassicas by protoplast fusion and hybrid recovery.

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Abbreviations

B5:

Gamborg et al., 1968

K3:

Kao and Michayluk, 1974

MS:

Murashige and Skoog, 1962

BAP:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

NAA:

1-naphthalene acetic acid

GA3 :

Gibberellic acid

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Authors and Affiliations

  1. Programme in Genetical Research, Bose Institute, P1/12, C.I.T. Scheme VII-M, 700 054, Calcutta, India

    Samir R. Sikdar, Gouri Chatterjee, Srabani Das & S. K. Sen

Authors
  1. Samir R. Sikdar
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  2. Gouri Chatterjee
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  3. Srabani Das
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  4. S. K. Sen
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Communicated by G.C. Phillips

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Sikdar, S.R., Chatterjee, G., Das, S. et al. Regeneration of plants from mesophyll protoplasts of the wild crucifer Eruca sativa Lam.. Plant Cell Reports 6, 486–489 (1987). https://doi.org/10.1007/BF00272790

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  • DOI: https://doi.org/10.1007/BF00272790

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