Abstract
Hairy root culture of Ginseng (Panax ginseng) was established after roots were induced on callus following infection with Agrobacterium rhizogenes. The transformed cultures of ginseng could be subcultured as an axenic root culture in the absence of phytohormones, and grew with extensive lateral branches more rapidly than the ordinary cultured roots induced by hormonal control from ginseng callus. The hairy roots synthesized the same saponins, ginsenosides, as those of the native root, up to about 2.4 times in the quantity, and up to about 2 times in comparison with that of the ordinary cultured roots, on dry weight basis.
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Abbreviations
- Ms medium:
-
Murashige and Skoog's medium
- 2,4-D:
-
2,4-dichloro-phenoxyacetic acid
- IBA:
-
indole-3-butyric acid
- K:
-
kinetin
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Communicated by F. Constabel
This paper is Part 52 in the series "Studies on Plant Tissue Culture". For Part 51, see Ayabe S, Udagawa A, Furuya T (1987).
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Yoshikawa, T., Furuya, T. Saponin production by cultures of Panax ginseng transformed with Agrobacterium rhizogenes . Plant Cell Reports 6, 449–453 (1987). https://doi.org/10.1007/BF00272780
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DOI: https://doi.org/10.1007/BF00272780