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Transformation of lettuce (Lactuca sativa) mediated by Agrobacterium tumefaciens

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Abstract

Lactuca sativa can be routinely transformed using Ti plasmids of Agrobacterium tumefaciens containing a chimeric kanamycin resistance gene (NOS.NPTII.NOS). Critical experimental variables were plant genotype, bacterial concentration, presence of a nurse culture and timing of transfers between tissue culture media. Transformation was confirmed by the ability to callus and root in the presence of kanamycin, nopaline production, and by hybridization in Southern blots. Transformation has been achieved with several Ti vectors. Several hundred transformed plants have been regenerated. Kanamycin resistance was inherited monogenically. Homozygotes can be selected by growing R2 seedlings on media containing G418.

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Abbreviations

IAA:

indole acetic acid

KIN:

kinetin

BA:

benzyladenine

NOS:

nopaline synthase

NPTII:

neomycin phosphotransferase II

RMNO:

tobacco nutrient medium (Marton and Maliga, 1975)

SH:

Shenk & Hildebrandt nutrient medium (Shenk & Hildebrandt, 1972; Michelmore and Eash, 1985)

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Authors and Affiliations

  1. Department of Vegetable Crops, University of California, 95616, Davis, CA, USA

    Richard Michelmore, Ellen Marsh, Susan Seely & Benoit Landry

Authors
  1. Richard Michelmore
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  2. Ellen Marsh
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  3. Susan Seely
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  4. Benoit Landry
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Additional information

Communicated by L. K. Grill

Present address: Agriculture Canada, P.O. Box 457, St. Jean-sur-Richelieu, Quebec, Canada, J3B 6Z8

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Michelmore, R., Marsh, E., Seely, S. et al. Transformation of lettuce (Lactuca sativa) mediated by Agrobacterium tumefaciens . Plant Cell Reports 6, 439–442 (1987). https://doi.org/10.1007/BF00272777

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  • DOI: https://doi.org/10.1007/BF00272777

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